Background

One of the important factors in the occurrence of acute kidney injury (AKI) among renal transplant patients (RTPs) is ischemia reperfusion injury (IRI). The current study aimed at determining the anti-inflammatory and anti-oxidative effects of melatonin on the complications of IRI and the level of Klotho expression in these patients.

Methods

A total of 40 renal transplant candidates were randomly assigned into placebo or melatonin group receiving the same dose of 3 mg/day. In order to measure serum melatonin levels, inflammatory and oxidative stress factors, renal function biomarkers, and Klotho gene/protein expression, venous blood samples were taken from patients over two different time points, i e, 24 h before the transplantation and at discharge from hospital.

Results

Melatonin was associated with improvement in renal transplantation, since the serum level of neutrophil gelatinase-associated lipocalin, as a renal functional marker, significantly decreased (P < .001). The effect of melatonin as a suppressor of inflammation and oxidative stress was also evident in the melatonin group due to a significant reduction in the serum levels of MDA, CP, 8-OHdG, and TNF-α markers (P < .001).

Conclusions

Reduction in serum levels of renal function and oxidative stress/inflammatory markers in the melatonin group indicates that melatonin can inhibit IRI outcomes in RTPs through its anti-oxidant and anti-inflammatory properties. However, these properties do not appear as a result of influence on the level of Klotho gene/protein expression.

中文翻译：

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口服褪黑激素对移植患者肾脏缺血再灌注损伤的影响：一项双盲，随机对照试验。

背景

在肾移植患者（RTP）中发生急性肾损伤（AKI）的重要因素之一是缺血再灌注损伤（IRI）。当前的研究旨在确定褪黑激素对这些患者的IRI并发症和Klotho表达水平的抗炎和抗氧化作用。

方法

总共将40名肾移植候选者随机分配至接受相同剂量3 mg /天的安慰剂或褪黑激素组。为了测量血清褪黑激素水平，炎性和氧化应激因素，肾功能生物标志物以及Klotho基因/蛋白质表达，在两个不同的时间点（即移植前24小时和出院时）从患者那里采集静脉血样本。

结果

褪黑激素与肾脏移植的改善有关，因为嗜中性粒细胞明胶酶相关的脂质运载蛋白的血清水平（作为肾功能标记物）显着降低（P  <.001）。褪黑激素作为炎症和氧化应激的抑制剂的作用在褪黑激素组中也很明显，这是由于MDA，CP，8-OHdG和TNF-α标记物的血清水平显着降低（P  <.001）。

结论

褪黑激素组的血清肾功能和氧化应激/炎症标志物水平降低表明褪黑激素可通过其抗氧化和抗炎特性抑制RTP中的IRI结果。但是，这些属性并未显示出对Klotho基因/蛋白质表达水平的影响。