BACKGROUND The objective of this study was to report an outbreak of human toxoplasmosis occurring in the municipality of Montes Claros de Goiás, Goiás, Brazil, from December 2015 to August 2016. Seven acute cases in June 2016 triggered the subsequent search. METHODS A total of 251 individuals were selected through an active search, of which 114 (45.4%) agreed to participate in the research and blood collection. For serological diagnosis were used enzyme-linked immunosorbent assay (ELISA) for IgG and IgM and avidity tests. RESULTS Of the 114 serum samples evaluated, 12.3% (14/114) showed antibodies against Toxoplasma gondii, with a profile indicative of acute infection. Samples of artisan fresh cheese, public water, vegetables and irrigation water were collected. Toxoplasma gondii DNA fragments were amplified using the polymerase chain reaction from two samples of artisan fresh cheese and a sample of irrigation water from the vegetable garden. A control case study was carried out, and the variable cow's artisan fresh cheese consumption was statistically significant (p = .01). CONCLUSIONS The results showed that cheese analysed and/or irrigated water of vegetable represented an important route of transmission for the disease. This is the first reported outbreak possibly caused by cow's artisan fresh cheese. It is difficult to prove that these routes were the cause of the outbreak; however, the findings allow us to infer that the individuals involved in the outbreak were in contact with these risk factors.

中文翻译：

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在巴西弓形虫病暴发中，工匠用新鲜牛奶制成的新鲜奶酪是可能的传播途径。

背景技术这项研究的目的是报告2015年12月至2016年8月在巴西戈亚斯市Montes Claros deGoiás市发生的人弓形虫病暴发。2016年6月的7例急性病例引发了随后的搜寻。方法通过主动搜索共选择了251名个体，其中114名（45.4％）同意参加研究和采血。对于血清学诊断，使用酶联免疫吸附测定（ELISA）进行IgG和IgM以及亲和力测试。结果在所评估的114个血清样本中，有12.3％（14/114）显示了针对弓形虫的抗体，其特征表明存在急性感染。收集了工匠新鲜奶酪，公共用水，蔬菜和灌溉用水的样品。使用聚合酶链反应从两个工匠新鲜奶酪样品和一个菜园中的灌溉用水样品中扩增弓形虫DNA片段。进行了对照案例研究，并且可变的母牛的工匠新鲜奶酪的消费量具有统计学意义（p = 0.01）。结论结果表明，奶酪分析和/或灌溉水是该疾病的重要传播途径。这是首次报道的爆发，可能是由牛工匠的新鲜奶酪引起的。很难证明这些路线是爆发的原因。但是，调查结果使我们能够推断出与疾病爆发有关的个体与这些危险因素有关。