The diverse roles of cellular RNAs can be studied by purifying RNAs of interest together with the biomolecules they bind. Biotinylated antisense oligonucleotides that hybridize specifically to the RNA of interest provide a general approach to develop affinity reagents for these experiments. Such oligonucleotides can be used to enrich endogenous RNAs from cross-linked chromatin extracts to study the genomic binding sites of RNAs. These hybridization capture protocols are evolving modular experiments that are compatible with a range of cross-linkers and conditions. This review discusses the principles of these hybridization capture experiments as well as considerations and controls necessary to interpret the resulting data without being misled by artifactual signals.

中文翻译：

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研究作用于染色质的长链非编码 RNA 的杂交捕获实验的原理和实践。

可以通过纯化感兴趣的 RNA 及其结合的生物分子来研究细胞 RNA 的不同作用。与目标 RNA 特异性杂交的生物素化反义寡核苷酸为开发用于这些实验的亲和试剂提供了一种通用方法。此类寡核苷酸可用于从交联染色质提取物中富集内源性 RNA，以研究 RNA 的基因组结合位点。这些杂交捕获协议正在发展与一系列交联剂和条件兼容的模块化实验。这篇综述讨论了这些杂交捕获实验的原理，以及在不被人为信号误导的情况下解释结果数据所需的注意事项和控制。