Abstract This research investigated the antiproliferative effects of 1–500 μM fisetin in T98G and BEAS-2B cells by MTT assay. The IC50 of fisetin in T98G cells for 24 and 48 h were 93 and 75 μM, respectively. Apoptotic alterations of fisetin-treated T98G cells were observed by transmission electron microscopy. BEAS-2B was then used in comparison to T98G cells to determine the cytotoxic effects of fisetin. The IC50 of fisetin for 24 and 48 h were recorded as 270 and 90 μM in BEAS-2B cells, respectively. Different concentrations of fisetin were selected to determine the apoptotic and necrotic effects. Consequently, fisetin was determined to have more apoptotic effects in T98G than BEAS-2B cells, dose- and time-dependently. Moreover, fisetin was found to have cytotoxicity at lower doses in T98G cells compared to carmustine, as positive control. CASPASE 3, CASPASE 9, CASPASE 8, and BAX expressions were increased by the selected fisetin doses of 25 and 50 μM, while that of BCL-2 and survivin was reduced in T98G cells. These results will serve as an essential basis of future in vitro and in vivo studies, in the continuous search for alternative treatment agents for gliomas.

中文翻译：

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非瑟酮对胶质瘤细胞增殖和凋亡的影响

摘要 本研究通过 MTT 法研究了 1-500 μM 非瑟酮对 T98G 和 BEAS-2B 细胞的抗增殖作用。T98G 细胞中漆黄素在 24 和 48 小时内的 IC50 分别为 93 和 75 μM。通过透射电子显微镜观察非瑟酮处理的 T98G 细胞的凋亡改变。然后使用 BEAS-2B 与 T98G 细胞进行比较以确定非瑟酮的细胞毒性作用。在 BEAS-2B 细胞中，fisetin 24 和 48 小时的 IC50 分别记录为 270 和 90 μM。选择不同浓度的非瑟酮来确定细胞凋亡和坏死作用。因此，确定非瑟酮在 T98G 中比 BEAS-2B 细胞具有更多的凋亡效应，剂量和时间依赖性。此外，与作为阳性对照的卡莫司汀相比，发现非瑟酮在较低剂量的 T98G 细胞中具有细胞毒性。CASPASE 3、CASPASE 9、CASPASE 8 和 BAX 的表达通过选择的 25 和 50 μM 非瑟酮剂量增加，而 BCL-2 和存活蛋白在 T98G 细胞中的表达减少。这些结果将作为未来体外和体内研究的重要基础，不断寻找神经胶质瘤的替代治疗剂。