To identify flystrike-related volatile compounds in wool from Merino sheep, the attractiveness of wool to Lucilia cuprina Wiedmann (Diptera: Calliphoridae) was examined. First, a selection of wool samples guided by previous knowledge of sheep lines, predicted to be more susceptible or more resistant to flystrike, was tested. The attractiveness of the 10 samples selected was not associated with field susceptibility: two samples from the more resistant line were identified as most attractive and two samples from the more susceptible line were identified as least attractive, based on the behavioural assays with gravid flies. Comparison of the headspace volatiles of these samples, using solid phase microextraction and gas chromatography-mass spectrometry-electroantennographic detection, revealed octanal and nonanal to be present in the attractive wool samples that elicited responses from the fly antenna. Furthermore, the two compounds were not present in wool that was least attractive to L. cuprina. In laboratory bioassays, octanal and nonanal evoked antennal and behavioural responses in gravid L. cuprina, thus confirming their potential role as semiochemicals responsible for attracting L. cuprina to Merino sheep.

中文翻译：

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美利奴羊毛的挥发物引起了澳大利亚绵羊吹蝇卢西莉亚铜杯的触角和行为反应。

为了鉴定美利奴绵羊羊毛中与雷击有关的挥发性化合物，研究了羊毛对Lucilia cuprina Wiedmann（Diptera：Calliphoridae）的吸引力。首先，测试了根据先前对绵羊品系的了解而选择的羊毛样本，这些羊毛样本预计更易受打击或对中风有更强的抵抗力。所选择的10个样品的吸引力与田间药敏性无关：根据妊娠蝇的行为分析，将更具抗性的品系中的两个样品鉴定为最有吸引力，而来自较易感品系中的两个样品鉴定为最不有吸引力。使用固相微萃取和气相色谱-质谱-电造影检测这些样品的顶空挥发物，揭示了在有吸引力的羊毛样品中存在辛酸和壬醛，这些样品引起了苍蝇触角的响应。此外，这两种化合物都不存在于对L. cuprina吸引力最小的羊毛中。在实验室生物测定中，羊肚和壬草诱发了妊娠丘比特乳杆菌的触角和行为反应，从而证实了它们作为负责将丘比特乳杆菌吸引到美利奴绵羊的信息化学物质的潜在作用。