The spliceosome is a highly complex, dynamic ribonucleoprotein molecular machine that undergoes numerous structural and compositional rearrangements that lead to the formation of its active site. Recent advances in cyroelectron microscopy (cryo-EM) have provided a plethora of near-atomic structural information about the inner workings of the spliceosome. Aided by previous biochemical, structural, and functional studies, cryo-EM has confirmed or provided a structural basis for most of the prevailing models of spliceosome function, but at the same time allowed novel insights into splicing catalysis and the intriguing dynamics of the spliceosome. The mechanism of pre-mRNA splicing is highly conserved between humans and yeast, but the compositional dynamics and ribonucleoprotein (RNP) remodeling of the human spliceosome are more complex. Here, we summarize recent advances in our understanding of the molecular architecture of the human spliceosome, highlighting differences between the human and yeast splicing machineries.

中文翻译：

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对高等真核生物中核前体 mRNA 剪接的结构洞察。

剪接体是一种高度复杂的动态核糖核蛋白分子机器，它经历了许多结构和组成重排，导致其活性位点的形成。冷冻电子显微镜 (cryo-EM) 的最新进展提供了大量关于剪接体内部工作的近原子结构信息。借助先前的生化、结构和功能研究，cryo-EM 已经证实或为大多数流行的剪接体功能模型提供了结构基础，但同时也为剪接催化和剪接体的有趣动力学提供了新的见解。前体 mRNA 剪接机制在人类和酵母之间高度保守，但人类剪接体的组成动力学和核糖核蛋白 (RNP) 重塑更为复杂。这里，