One of the major problems with the formulation of herbal medicines is the quality control of plant material to ensure its efficacy and safety. Quality control of medicinal plants requires analysis of many bioactive compounds present in the plant. C-alkylated flavonoids are an important bioactive subclass of flavonoids. A simple, rapid, sensitive and selective method is presented here for the quantification of bioactive C-alkylated flavonoids. This is the first quantitative method for analysis of C-alkylated flavonoids based on the multiple reaction monitoring (MRM) approach so far. This study focuses on method development for quantification of bioactive C-alkylated flavonoids. Quantification of a total of five C-alkylated flavonoids was done employing the MRM approach on an HPLC-QqQ-MS instrument. LODs and LOQs for quantified flavonoids were in the range of 0.41-1.32 and 1.23-3.96 ng/mL, respectively. Linear calibration curves between 25 and 1500 ng/mL were obtained with the regression coefficients of ≥0.996. Accuracy (% bias) and precision (% RSD) of the analyses were found to be less than 5%. Developed HPLC-ESI-MS/MS can be employed as a quality control method of plant raw materials.

中文翻译：

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使用多反应监测方法通过HPLC-ESI-MS / MS灵敏测定C-烷基化类黄酮：假单胞菌为案例研究。

草药制剂的主要问题之一是植物材料的质量控制，以确保其功效和安全性。药用植物的质量控制需要分析植物中存在的许多生物活性化合物。C-烷基化的类黄酮是类黄酮的重要生物活性亚类。本文介绍了一种简单，快速，灵敏和选择性的方法，用于定量生物活性C-烷基化类黄酮。到目前为止，这是基于多反应监测（MRM）方法分析C-烷基化类黄酮的第一种定量方法。这项研究专注于定量生物活性C-烷基化类黄酮的方法开发。在HPLC-QqQ-MS仪器上采用MRM方法对总共5个C-烷基化的类黄酮进行了定量。定量的类黄酮的LOD和LOQ分别在0.41-1.32和1.23-3.96 ng / mL的范围内。获得的线性校准曲线在25至1500 ng / mL之间，回归系数≥0.996。分析的准确性（偏差百分比）和精度（RSD百分比）小于5％。所开发的HPLC-ESI-MS / MS可用作植物原料的质量控制方法。