Abstract This is the first report exploring the capability of twisted intercalating nucleic acid (TINA) and naphthalene-functionalized non-nucleosidic linkers to stabilize and engage in double-helical structures. Four designs were studied with respect to the formation of duplexes and/or other types of self-assemblies. One of the constructs involving TINA provides a thermostable duplex. The biophysical properties of the individual constructs were investigated by UV thermal melting experiments, circular dichroism, and fluorescence emission spectroscopy. Molecular modeling studies were performed in attempts of explaining the biophysical measurements for the duplex based on the TINA-containing oligonucleotide strands. Graphical abstract

中文翻译：

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基于包含嵌入核酸单体的寡核苷酸的新型组装

摘要 这是第一份探索扭曲嵌入核酸 (TINA) 和萘功能化非核苷连接体稳定和参与双螺旋结构的能力的报告。关于双链体和/或其他类型的自组装的形成研究了四种设计。涉及 TINA 的构建体之一提供了热稳定双链体。通过紫外热熔实验、圆二色性和荧光发射光谱研究了单个构建体的生物物理特性。为了解释基于含 TINA 的寡核苷酸链的双链体的生物物理测量，进行了分子建模研究。图形概要