BACKGROUND Gastrointestinal (GI) hemorrhage accompanies several common diseases of rhesus macaques (Macaca mulatta). Guaiac fecal occult blood testing (gFOBT) is a non-invasive means to detect such bleeding in several species; however, there are currently no data indicating reliability of this test to detect GI hemorrhage in macaques. METHODS We evaluated sensitivity and specificity of gFOBT to detect simulated and biopsy-associated bleeding in the stomach, duodenum, and colon of 15 rhesus macaques. Fecal samples were analyzed via gFOBT for 72 hours. RESULTS Guaiac fecal occult blood testing was more sensitive to detect lower vs upper GI bleeding; sensitivity was volume-dependent in the upper GI tract. Single-test specificity was 95.2%. Repeated fecal collections increased gFOBT sensitivity without affecting specificity. CONCLUSIONS Guaiac fecal occult blood testing is a useful screening test for both upper and lower GI bleeding in rhesus macaques. For highest sensitivity, gFOBT should be performed on three fecal samples collected 24 hours apart.

中文翻译：

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愈创木脂粪便潜血试验检测恒河猴（Macaca mulatta）胃肠道出血的评估。


背景技术 胃肠道（GI）出血伴随着恒河猴（Macaca mulatta）的几种常见疾病。愈创木脂粪便潜血检测 (gFOBT) 是一种非侵入性方法，可检测多种物种的此类出血情况；然而，目前没有数据表明该测试检测猕猴胃肠道出血的可靠性。方法 我们评估了 gFOBT 检测 15 只恒河猴胃、十二指肠和结肠模拟出血和活检相关出血的敏感性和特异性。通过 gFOBT 分析粪便样本 72 小时。结果 愈创木脂粪便潜血检测对于检测下消化道出血比上消化道出血更敏感；上消化道的敏感性取决于体积。单次检测特异性为95.2%。重复粪便采集可增加 gFOBT 敏感性，而不影响特异性。结论 愈创木脂粪便潜血试验是恒河猴上消化道和下消化道出血的有效筛查试验。为获得最高灵敏度，应对间隔 24 小时收集的三份粪便样本进行 gFOBT。