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Nuclear pore protein Nup98 is involved in replication of Rift Valley fever virus and nuclear import of virulence factor NSs.
Journal of General Virology ( IF 3.6 ) Pub Date : 2020-07-01 , DOI: 10.1099/jgv.0.001347
Simone Lau 1, 2 , Friedemann Weber 1, 2, 3
Affiliation  

The non-structural protein NSs is the main virulence factor of Rift Valley fever virus, a major zoonotic pathogen in Africa. NSs forms large aggregates in the nucleus and impairs induction of the antiviral type I IFN system by several mechanisms, including degradation of subunit p62 of the general RNA polymerase II transcription factor TFIIH. Here, we show that depletion of the nuclear pore protein Nup98 affects the nuclear import of NSs. Nonetheless, NSs was still able to degrade TFIIH-p62 under these conditions. Depletion of Nup98, however, had a negative effect on Rift Valley fever virus multiplication. Our data thus indicate that NSs utilizes Nup98 for import into the nucleus, but also plays a general role in the viral replication cycle.

中文翻译:


核孔蛋白 Nup98 参与裂谷热病毒的复制和毒力因子 NS 的核输入。



非结构蛋白NSs是非洲主要人畜共患病原体裂谷热病毒的主要毒力因子。 NS 在细胞核中形成大的聚集体,并通过多种机制损害抗病毒 I 型 IFN 系统的诱导,包括降解通用 RNA 聚合酶 II 转录因子 TFIIH 的亚基 p62。在这里,我们证明核孔蛋白 Nup98 的消耗会影响 NS 的核输入。尽管如此,NSs 在这些条件下仍然能够降解 TFIIH-p62。然而,Nup98 的缺失对裂谷热病毒的增殖产生负面影响。因此,我们的数据表明 NS 利用 Nup98 输入细胞核,但也在病毒复制周期中发挥一般作用。
更新日期:2020-08-20
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