Summary The present study proposes a new methodology for the quantification of parasite eggs in animal tissue. Quantification of parasites are important to understand epidemiology of spirorchiid infections in sea turtles, however different methodologies for quantifying Spirorchiidae eggs in turtle tissues have been used. The most representative way to quantify Spirorchiidae burdens in tissues is counting eggs / g of tissue, however, this method is very laborious. As an alternative, we propose quantifying number of Spirorchiidae eggs/ area of tissue on a microscope slide. We compared this method to number of eggs / slide, a common metric of egg burden in turtle tissues. Both methods correlated well with eggs / g with eggs/mm2 of tissue having better correlation.

中文翻译：

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量化绿海龟脾脏组织学样本中的螺旋体卵

总结 本研究提出了一种量化动物组织中寄生虫卵的新方法。寄生虫的量化对于了解海龟螺旋体感染的流行病学很重要，但是已经使用了不同的方法来量化海龟组织中的螺旋体科卵。量化组织中螺旋体科负担的最具代表性的方法是计算鸡蛋/克组织，但是，这种方法非常费力。作为替代方案，我们建议量化显微镜载玻片上的螺旋体科卵数/组织面积。我们将此方法与鸡蛋/载玻片的数量进行了比较，这是一种衡量海龟组织中鸡蛋负担的常用指标。两种方法与鸡蛋/g 的相关性很好，而鸡蛋/mm2 的组织具有更好的相关性。