Summary Echinococosis is a zoonotic disease caused by the larval stages of Echinococcus spp. that occurs in most parts of the world. Herein, we aimed to evaluate the genotypes of isolated hydatid cysts from slaughtered animals in Shush county, southwestern Iran. Totally, 96 hydatid cysts were collected, including 11 buffaloes, 13 cattle, 12 goat and 60 sheep. The PCR was done by a primer pair (BDI and 4s) to amplify ITS1 fragment. Four restriction endonucleases including AluI, HpaII, RsaI, and TaqI were used for RFLP products and enzymatic reactions were electrophoresed. Finally, twenty PCR products were sent for sequencing and phylogenetic tree was drawn with MEGA6. Molecular identification of 96 hydatid cysts demonstrated a distinctive 1000 bp fragment in all samples from four animal hosts. RFLP analysis showed similar digestion patterns in all samples. AluI digestion yielded 800 bp and 200 bp fragments, HpaII digestion made 700 bp and 300 bp fragments and RsaI digestion entailed 655 and 345segments. Moreover, TaqI rendered no digestion pattern on rDNA-ITS1 region. Additionally, E. granulosus sensu stricto (G1-3 complex) was the prevailing genotype in all livestock samples, according to PCR-RFLP and sequencing analyses.

中文翻译：

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PCR-RFLP法测定伊朗西南部舒什县屠宰家畜中细粒棘球绦虫基因型

总结 包虫病是一种由棘球绦虫幼虫阶段引起的人畜共患疾病。这发生在世界大部分地区。在此，我们旨在评估伊朗西南部舒什县屠宰动物的分离包虫包囊的基因型。共采集到包虫包囊96个，其中水牛11头，牛13头，山羊12头，绵羊60头。PCR由引物对（BDI和4s）完成以扩增ITS1片段。包括 AluI、HpaII、RsaI 和 TaqI 在内的四种限制性内切核酸酶用于 RFLP 产品，并对酶促反应进行电泳。最后将20个PCR产物送去测序，用MEGA6绘制系统进化树。96 个包虫包囊的分子鉴定表明在来自四个动物宿主的所有样品中都有一个独特的 1000 bp 片段。RFLP 分析显示所有样品中的消化模式相似。AluI 消化产生 800 bp 和 200 bp 片段，HpaII 消化产生 700 bp 和 300 bp 片段，RsaI 消化需要 655 和 345 个片段。此外，TaqI 在 rDNA-ITS1 区域没有呈现消化模式。此外，根据 PCR-RFLP 和测序分析，狭义细粒大肠杆菌（G1-3 复合体）是所有牲畜样本中的主要基因型。