Summary The control of the digenetic trematode Fasciola gigantica has been the major challenge in both cattle and small ruminants as there is a paucity of an effective and commercial vaccine. Thus, the accurate identification and prepatent diagnosis of F. gigantica is an essential prerequisite for its successful prevention and control. In the present study, the morphologically identified specimens isolated from the liver and bile ducts of sheep (Ovis aries) were validated through molecular data. The sequence analysis of ITS-2 of our isolates showed high degree of similarity with F. gigantica and F. hepatica using BLAST function of NCBI. The phylogenetic analysis of our isolates showed a close relationship with previously described F. gigantica and F. hepatica isolates from different countries. The antigenic profile of somatic and E/S antigens of F. gigantica were revealed by SDS–PAGE and immunoblotting using sera from sheep naturally infected with F. gigantica. By SDS-PAGE, 20 distinct bands were revealed from crude somatic fraction. Immunoblotting analysis of these proteins with positive sera exhibited 8 sero-reactive bands ranging from 14 to 97 kDa. Among these 38 and 44 kDa bands were quite specific with high diagnostic specificity and sensitivity. The E/S fraction comprised 7 distinct bands, as revealed by SDS-PAGE analysis. Immunoblotting analysis of these proteins with positive sera exhibited 6 antigenic bands ranging from 23 – 54 kDa. Among these 27 and 33 kDa were found to be quite specific with high diagnostic specificity and sensitivity. The present study concludes that the protein bands of 38 and 44 kDa in somatic fraction and 27 and 33 kDa in E/S fraction can be used for the immunodiagnostic purpose for this economically important parasite, which may also entice further studies regarding their vaccine potential.

中文翻译：

---

从喜马拉雅西北地区绵羊中分离出的巨大片形吸虫各种抗原蛋白的分子表征和免疫诊断潜力

总结 由于缺乏有效的商业疫苗，控制双遗传吸虫巨大片形吸虫一直是牛和小反刍动物面临的主要挑战。因此，准确识别和预先诊断巨型镰刀菌是其成功防控的必要前提。在本研究中，通过分子数据验证了从绵羊（Ovis aries）的肝脏和胆管中分离出的形态学鉴定标本。使用 NCBI 的 BLAST 功能，我们分离株的 ITS-2 的序列分析显示与 F. gigantica 和 F. livera 高度相似。我们分离株的系统发育分析表明与先前描述的来自不同国家的 F. gigantica 和 F. livera 分离株密切相关。F. 体细胞和 E/S 抗原的抗原谱。使用来自自然感染 F. gigantica 的绵羊的血清，通过 SDS-PAGE 和免疫印迹揭示了 gigantica。通过SDS-PAGE，从粗体细胞级分中揭示了20条不同的条带。这些具有阳性血清的蛋白质的免疫印迹分析显示出 8 个血清反应带，范围从 14 到 97 kDa。在这些 38 和 44 kDa 条带中，具有很高的诊断特异性和灵敏度。E/S 部分包含 7 个不同的条带，如 SDS-PAGE 分析所示。这些具有阳性血清的蛋白质的免疫印迹分析显示出 6 个抗原条带，范围为 23 – 54 kDa。其中 27 和 33 kDa 被发现具有很高的诊断特异性和敏感性。