This commentary discusses particularities of application of the EuroFlow standardization of flow cytometric analyses on three different flow cytometers. The EuroFlow consortium developed a fully standardized approach for flow cytometric immunophenotyping of hematological malignancies and primary immunodeficiencies. Standardized instrument setup is an essential part of EuroFlow standardization. Initially, the EuroFlow Consortium developed and optimized a step-by-step standard operating procedure (SOP) to setup 8-color BD FACSCanto II flow cytometer (Canto), with the later inclusion of Navios (Beckman Coulter) and BD FACSLyric (Lyric). Those SOPs were developed to enable standardized and fully comparable fluorescence measurements in the three flow cytometers. In Canto and Navios, mean fluorescence intensity (MFI) of a reference peak of Rainbow beads calibration particles is used to set up photomultiplier (PMT) voltages for each detector channel in individual instruments to reach the same MFI across distinct instruments. In turn, a new feature of Lyric instruments allows to share collection of attributes that are used to place the positive population at the same position among instruments in the form of assays, as one of its components integrated in the Cytometer Setup and Tracking (CS&T) module. The EuroFlow Lyric assays thus allow for standardized acquisition of 8-color EuroFlow panels on Lyric without the need to setup the PMT voltages on the individual instruments manually.

In summary, the standardized instrument setup developed by EuroFlow enables cross-platform inter- and intra-laboratory standardization of flow cytometric measurements. This commentary provides a perspective on the modifications of the standardized EuroFlow instrument setup of Canto, Navios and Lyric instruments that are described in detail in individual instrument-specfic SOPs available at the EuroFlow website.

本评论讨论了流式细胞仪分析的 EuroFlow 标准化在三种不同流式细胞仪上应用的特殊性。EuroFlow 联盟开发了一种完全标准化的方法，用于血液系统恶性肿瘤和原发性免疫缺陷的流式细胞免疫表型分析。标准化仪器设置是 EuroFlow 标准化的重要组成部分。最初，EuroFlow 联盟开发并优化了逐步标准操作程序 (SOP) 以设置 8 色 BD FACSCanto II 流式细胞仪 (Canto)，后来包括 Navios (Beckman Coulter) 和 BD FACSLyric (Lyric) . 开发这些 SOP 是为了在三个流式细胞仪中实现标准化和完全可比的荧光测量。在 Canto 和 Navios 中，Rainbow 珠子校准粒子参考峰的平均荧光强度 (MFI) 用于为各个仪器中的每个检测器通道设置光电倍增管 (PMT) 电压，以在不同仪器中达到相同的 MFI。反过来，Lyric 仪器的一项新功能允许共享属性集合，这些属性用于以分析的形式将阳性群体置于仪器之间的同一位置，作为集成在细胞仪设置和跟踪 (CS&T) 中的组件之一模块。因此，EuroFlow Lyric 检测允许在 Lyric 上标准化采集 8 色 EuroFlow 面板，而无需手动设置各个仪器上的 PMT 电压。

总之，EuroFlow 开发的标准化仪器设置可实现流式细胞仪测量的跨平台实验室间和实验室内标准化。本评论提供了对 Canto、Navios 和 Lyric 乐器的标准化 EuroFlow 乐器设置修改的看法，这些修改在 EuroFlow 网站上提供的各个乐器特定 SOP 中进行了详细描述。