At present, the planet faces a change in the composition and bioavailability of nutrients. Zinc deficiency is a widespread problem throughout the world. It is imperative to understand the mechanisms that organisms use to adapt to the deficiency of this micronutrient. In the Ascomycetes fungi, the ZIP family of proteins is one of the most important for zinc transport and includes high affinity Zrt1p and low zinc affinity Zrt2p transporters. After identification and characterization of ZRT1/ZRT2-like genes in Ustilago maydis we conclude that they encode for high and low zinc affinity transporters, with no apparent iron transport activity. These conclusions were supported by the gene deletion in Ustilago and the functional characterization of ZRT1/ZRT2-like genes by measuring the intracellular zinc content over a range of zinc availability. The functional complementation of the S. cerevisiae ZRT1Δ ZRT2Δ mutant with U. maydis genes supports this as well. U. maydis ZRT2 gene, was found to be regulated by pH through Rim101 pathway, thus providing novel insights into how this Basidiomycota fungus can adapt to different levels of Zn availability.

中文翻译：

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担子菌门中的锌吸收：玉米黑粉菌中锌转运蛋白的表征。

目前，地球面临着营养成分和生物利用度的变化。缺锌是全世界普遍存在的问题。必须了解生物体适应这种微量营养素缺乏的机制。在子囊菌中，ZIP 蛋白家族是锌转运最重要的蛋白之一，包括高亲和力 Zrt1p 和低锌亲和力 Zrt2p 转运蛋白。在对玉米黑粉菌中的 ZRT1/ZRT2 样基因进行鉴定和表征后，我们得出结论，它们编码高和低锌亲和力转运蛋白，没有明显的铁转运活性。这些结论得到了黑粉菌基因删除以及通过测量一定锌利用率范围内的细胞内锌含量来表征 ZRT1/ZRT2 样基因的功能特征的支持。酿酒酵母 ZRT1Δ ZRT2Δ 突变体与玉蜀黍基因的功能互补也支持了这一点。玉米 ZRT2 基因被发现通过 Rim101 途径受 pH 调节，从而为这种担子菌门真菌如何适应不同水平的锌可用性提供了新的见解。