Chromium (Cr) is commonly added into various metal alloys to improve some mechanical properties such as corrosion resistance, strength, and workability. However, Cr is also known to be a metal allergen for some individuals. Metal allergy is a T cell-mediated disease with symptoms of inflammation and swelling that involve inflammatory cytokines and prostaglandins. Hence, suppressing these inflammation paths by using COX-2 inhibitor might be useful in treating Cr allergy. In this study, mice were used with Cr-induced allergy challenge model. The mice were injected with celecoxib once per day for 7 days one hour after the challenge. Footpad samples were stained with haematoxylin and eosin (H&E), and lymphocytes were isolated from popliteal lymph nodes for the flow cytometric analysis. The results show that both prostaglandin E2 (PGE2), a known mediator of inflammation, and cyclooxygenases (COX)-2 have important roles in the development of Cr allergy. Further, COX-2 inhibitor, celecoxib, was effective in relieving swelling and inflammation in Cr-allergic mice concordant with suppression of IFN-γ production by CD8+ T cells and T cell accumulation in the lymph nodes. Therefore, the inhibition of COX-2 may be a therapeutic target for Cr allergy, and additional molecules in the PGE2 signalling pathway may also be an effective therapeutic target for the treatment of metal allergy.

中文翻译：

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在铬过敏发生期间，COX-2诱导T细胞积累和IFN-γ产生。

铬（Cr）通常添加到各种金属合金中，以改善某些机械性能，例如耐腐蚀性，强度和可加工性。但是，对于某些人来说，铬也是一种金属过敏原。金属过敏是由T细胞介导的疾病，具有炎症和肿胀症状，涉及炎症细胞因子和前列腺素。因此，通过使用COX-2抑制剂抑制这些炎症路径可能对治疗Cr过敏有用。在这项研究中，小鼠与Cr诱发的过敏反应模型一起使用。攻击后一小时，每天一次给小鼠注射塞来昔布，持续7天。足垫样品用苏木精和曙红（H＆E）染色，并从pop淋巴结中分离淋巴细胞用于流式细胞仪分析。结果表明，前列腺素E2（PGE2），已知的炎症介质，环氧合酶（COX）-2在Cr过敏的发生中起重要作用。此外，COX-2抑制剂塞来昔布可有效缓解Cr过敏小鼠的肿胀和炎症，这与抑制CD8 + T细胞产生的IFN-γ和抑制T细胞在淋巴结中的蓄积有关。因此，抑制COX-2可能是Cr过敏的治疗靶标，PGE2信号通路中的其他分子也可能是治疗金属过敏的有效治疗靶标。可以有效缓解Cr过敏小鼠的肿胀和炎症，这与抑制CD8 + T细胞产生的IFN-γ和T细胞在淋巴结中的蓄积有关。因此，抑制COX-2可能是Cr过敏的治疗靶标，PGE2信号传导途径中的其他分子也可能是治疗金属过敏的有效治疗靶标。可以有效缓解Cr过敏小鼠的肿胀和炎症，这与抑制CD8 + T细胞产生的IFN-γ和T细胞在淋巴结中的蓄积有关。因此，抑制COX-2可能是Cr过敏的治疗靶标，PGE2信号通路中的其他分子也可能是治疗金属过敏的有效治疗靶标。