Queen conch (Lobatus gigas) is a marine gastropod endemic to the Caribbean. This species is a cultural symbol, being a significant local food source and the second largest commercial fishery in the region. However, over-exploitation and natural habitat degradation have exerted high survival pressure on this species. This work aims to provide novel proteomic data to highlight the metabolism of the species and to provide an important tool for the understanding of queen conch biology and physiology. Herein, we profiled the whole proteome from 3 organs (gills, digestive gland and muscle) of L. gigas combining gel-free and gel-based techniques. Overall 420 clusters of proteins were identified corresponding to the minimum identification requirement of protein sequence redundancy. Gene ontology and KEGG analysis highlighted 59 metabolic pathways between identified proteins. The most relevant routes according to the number of sequences found per pathway were purine and thiamine metabolism, closely related to nucleotide and carbohydrate metabolism. We also emphasize the high number of proteins associated to the biosynthesis of antibiotics (93 proteins and a total of 28 enzymes), which were among the top-twenty pathways identified by KEGG analysis. The proteomics approach allowed the identification and description of putative markers of oxidative stress, xenobiotic metabolism, heat shock response and respiratory chain for the first time in the species, which could be extremely useful in future investigations for diagnosing and monitoring L. gigas population health.

中文翻译：

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女王海螺（Lobatus gigas）蛋白质组：海洋腹足动物生物学研究的重要工具。

女王海螺（Lobatus gigas）是加勒比海特有的海洋腹足动物。该物种是一种文化象征，是当地重要的食物来源和该地区第二大商业渔业。但是，过度开发和自然栖息地退化给该物种带来了很大的生存压力。这项工作旨在提供新颖的蛋白质组学数据，以突出该物种的新陈代谢，并为理解海螺女王生物学和生理学提供重要工具。在这里，我们从L. gigas的3个器官（腮，消化腺和肌肉）中提取了整个蛋白质组图结合了无凝胶技术和基于凝胶的技术。鉴定出总共420个蛋白质簇，对应于蛋白质序列冗余的最低鉴定要求。基因本体论和KEGG分析突出显示了已鉴定蛋白质之间的59种代谢途径。根据每个途径发现的序列数，最相关的途径是嘌呤和硫胺素代谢，与核苷酸和碳水化合物代谢密切相关。我们还强调了与生物合成抗生素相关的大量蛋白质（93种蛋白质和28种酶），这些蛋白质是KEGG分析确定的前二十种途径之一。蛋白质组学方法可以识别和描述氧化应激，异种生物代谢，L. gigas人口健康。