Described here are instructions for building and using an inexpensive automated microscope (AMi) that has been specifically designed for viewing and imaging the contents of multi-well plates. The X, Y, Z translation stage is controlled through dedicated software (AMiGUI) that is being made freely available. Movements are controlled by an Arduino-based board running grbl, and the graphical user interface and image acquisition are controlled via a Raspberry Pi microcomputer running Python. Images can be written to the Raspberry Pi or to a remote disk. Plates with multiple sample wells at each row/column position are supported, and a script file for automated z-stack depth-of-field enhancement is written along with the images. The graphical user interface and real-time imaging also make it easy to manually inspect and capture images of individual samples.

中文翻译：

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AMi：一种基于 GUI 的开源系统，用于对多孔板中的样品进行成像。

这里描述的是构建和使用廉价自动显微镜 (AMi) 的说明，该显微镜专为观察和成像多孔板的内容而设计。X、Y、Z 平移台通过免费提供的专用软件 (AMiGUI) 进行控制。运动由运行 grbl 的基于 Arduino 的板控制，图形用户界面和图像采集由运行 Python 的 Raspberry Pi 微型计算机控制。图像可以写入 Raspberry Pi 或远程磁盘。支持每行/列位置具有多个样品孔的板，并且用于自动 z 堆栈景深增强的脚本文件与图像一起写入。图形用户界面和实时成像还可以轻松手动检查和捕获单个样品的图像。