Interleukin (IL)-35 is confirmed as an important modulator in immune response. The aim of the study was to explore the role of IL-35 in the development of polymyositis/dermatomyositis (PM/DM). Ninety-five patients with PM/DM and 30 healthy controls (HCs) were recruited. Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Paque Plus density gradient centrifugation. Protein IL-35, IL-17, and tumor necrosis factor (TNF)-α levels were measured using enzyme-linked immunosorbent assay method. The IL-35 serum levels in PM/DM patients were significantly higher than those in HCs and were correlated with PM/DM-related features: disease activity, muscle damage, and interstitial lung disease (ILD). Exogenous IL-35 notably suppressed lipopolysaccharide-induced IL-17 and TNF-α production in PBMCs of patients with PM/DM. Elevated serum IL-35 levels could act as a disease activity biomarker and an indicator of ILD in PM/DM. IL-35 may play an anti-inflammatory role in PM/DM.

中文翻译：

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白细胞介素35：多发性肌炎/皮肌炎患者的血清生物标志物。

白介素（IL）-35被确认为免疫反应中的重要调节剂。该研究的目的是探讨IL-35在多发性肌炎/皮肌炎（PM / DM）发生中的作用。招募了95名PM / DM患者和30名健康对照（HCs）。通过Ficoll-Paque Plus密度梯度离心分离外周血单个核细胞（PBMC）。使用酶联免疫吸附法测定蛋白质IL-35，IL-17和肿瘤坏死因子（TNF）-α的水平。PM / DM患者的IL-35血清水平显着高于HCs，并且与PM / DM相关的特征相关：疾病活动，肌肉损伤和间质性肺病（ILD）。外源性IL-35显着抑制了PM / DM患者的PBMC中脂多糖诱导的IL-17和TNF-α的产生。血清IL-35水平升高可能是疾病活动性生物标志物和PM / DM中ILD的指标。IL-35可能在PM / DM中起抗炎作用。