Current methods to treat large soft-tissue defects mainly rely on autologous transfer of adipocutaneous flaps, a method that is often limited by donor site availability. Engineered vascularized adipose tissues can potentially be a viable and readily accessible substitute to autologous flaps. In this study, we engineered a small-scale adipose tissue with pre-patterned vasculature that enables immediate perfusion. Vessels formed after one day of perfusion and displayed barrier function after three days of perfusion. Under constant perfusion, adipose tissues remained viable and responded to lipoactive hormones insulin and epinephrine with lipid accumulation and loss, respectively. Adipocyte growth correlated inversely with distance away from the feeding vessel, as predicted by a Krogh-type model.

中文翻译：

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工程化的微型血管化脂肪，对脂活性激素的灌注有反应。

当前治疗大型软组织缺损的方法主要依靠自体皮脂瓣的转移，该方法通常受到供体部位可用性的限制。工程化的血管化脂肪组织可能是自体皮瓣的可行且容易获得的替代物。在这项研究中，我们设计了具有预先构图的脉管系统的小规模脂肪组织，可以立即进行灌注。灌注一天后形成血管，并在灌注三天后显示屏障功能。在持续的灌注下，脂肪组织仍然可以存活，并且对脂活素胰岛素和肾上腺素有反应，分别导致脂质堆积和丢失。如Krogh型模型所预测的，脂肪细胞的生长与远离饲养血管的距离成反比。