The removal of afferent input to the olfactory bulb by both cautery and chemical olfactory organ ablation in adult zebrafish results in a significant decrease in volume of the ipsilateral olfactory bulb. To examine the effects of deafferentation at a cellular level, primary output neurons of the olfactory bulb, the mitral cells, were investigated using retrograde tract tracing with fluorescent dextran using ex vivo brain cultures. Morphological characteristics including the number of major dendritic branches, total length of dendritic branches, area of the dendritic arbor, overall dendritic complexity, and optical density of the arbor were used to determine the effects of deafferentation on mitral cell dendrites. Following 8 weeks of permanent deafferentation there were significant reductions in the total length of dendritic branches, the area of the dendritic arbor, and the density of fine processes in the dendritic tuft. With 8 weeks of chronic, partial deafferentation there were significant reductions in all parameters examined, including a modified Sholl analysis that showed significant decreases in overall dendritic complexity. These results show the plasticity of mitral cell dendritic structures in the adult brain and provide information about the response of these output neurons following the loss of sensory input in this key model system.

中文翻译：

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成年斑马鱼嗅球中脱除咖啡因引起的二尖瓣细胞树突形态改变。

成年斑马鱼通过烧灼和化学嗅觉器官消融去除嗅觉球的传入输入会导致同侧嗅球体积的显着减少。为了在细胞水平上检查脱除咖啡因的效果，使用荧光葡聚糖使用离体脑培养物进行逆行迹线追踪，研究了嗅球的主要输出神经元（二尖瓣细胞）。形态学特征包括主要树突分支的数量，树突分支的总长度，树突乔木的面积，总树突复杂性和乔木的光密度被用来确定去咖啡力作用对二尖瓣细胞树突的影响。永久性脱除咖啡因8周后，树突分支的总长度显着减少，树突状乔木的面积，以及树突状簇中精细过程的密度。经过8周的慢性，部分脱咖啡因，所有检查的参数均显着降低，包括改良的Sholl分析，该分析显示总体树突复杂性显着降低。这些结果显示了成年大脑中二尖瓣细胞树突状结构的可塑性，并提供了有关此关键模型系统中感觉输入丢失后这些输出神经元反应的信息。