Decellularized human pericardium is under study as an allogenic material for cardiovascular applications. The effects of crosslinking on the mechanical properties of decellularized pericardium were determined with a uniaxial tensile test, and the effects of crosslinking on the collagen structure of decellularized pericardium were determined by multiphoton microscopy. The viability of human umbilical vein endothelial cells seeded on decellularized human pericardium and on pericardium strongly and weakly crosslinked with glutaraldehyde and with genipin was evaluated by means of an MTS assay. The viability of the cells, measured by their metabolic activity, decreased considerably when the pericardium was crosslinked with glutaraldehyde. Conversely, the cell viability increased when the pericardium was crosslinked with genipin. Coating both non-modified pericardium and crosslinked pericardium with a fibrin mesh or with a mesh containing attached heparin and/or fibronectin led to a significant increase in cell viability. The highest degree of viability was attained for samples that were weakly crosslinked with genipin and modified by means of a fibrin and fibronectin coating. The results indicate a method by which in vivo endothelialization of human cardiac allografts or xenografts could potentially be encouraged.

中文翻译：

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人脱细胞和交联的心包被生物活性分子组装。

脱细胞的人心包正在研究作为心血管应用的同种异体材料。通过单轴拉伸试验确定交联对去细胞心包膜力学性能的影响，并通过多光子显微镜测定交联对去细胞心包膜胶原结构的影响。通过MTS测定评估了在脱细胞的人心包上以及在心包上与戊二醛和京尼平强交联和弱交联的人脐静脉内皮细胞的活力。当心包膜与戊二醛交联时，以其代谢活性衡量的细胞活力显着下降。相反，当心包膜与Genipin交联时，细胞活力增加。用纤维蛋白网或用含有附着的肝素和/或纤连蛋白的网涂覆未修饰的心包膜和交联的心包膜导致细胞活力的显着增加。对于那些用genipin弱交联并通过纤维蛋白和纤连蛋白涂层修饰的样品，可以达到最高的生存力。结果表明，通过这种方法可以潜在地促进人心脏同种异体移植物或异种移植物的体内内皮化。