Because the collagen membrane lacks osteoinductivity, it must be modified with bioactive components to trigger rapid bone regeneration. In this study, we aimed to evaluate the bone regeneration effects of a collagen membrane chemically conjugated with stromal cell-derived factor-1 alpha (SDF-1α) in rat models. To this end, different collagen membranes from four groups including a control group with a Bio-Oss bone substitute + collagen membrane; physical adsorption group with Bio-Oss + SDF-1α physically adsorbed on the collagen membrane; chemical cross-linking group with Bio-Oss + SDF-1α chemically cross-linked to the collagen membrane; and cell-seeding group with Bio-Oss + bone marrow mesenchymal stem cells (BMSCs) seeded onto the collagen membrane were placed in critical-sized defect models using a guided bone regeneration technique. At 4 and 8 weeks, the specimens were analyzed by scanning electron microscopy, energy-dispersive x-ray spectroscopy, micro-computed tomography, and histomorphology analyzes. Furthermore, ectopic osteogenesis was examined by histological analysis with Von Kossa staining, with the samples counterstained by hematoxylin and eosin and immunohistochemical staining. The results showed that in the chemical cross-linking group and cell-seeding group, the bone volume fraction, bone surface area fraction, and trabecular number were significantly increased and showed more new bone formation compared to the control and physical adsorption groups. Von Kossa-stained samples counterstained with hematoxylin and eosin and subjected to immunohistochemical staining of 4-week implanted membranes revealed that the chemical cross-linking group had the largest number of microvessels. The collagen membrane chemically conjugated with SDF-1α to significantly promote new bone and microvessel formation compared to SDF-1α physical adsorption and showed similar effects on new bone formation as a BMSC seeding method. This study provided a cell-free approach for shortening the bone healing time and improving the success rate of guided bone regeneration.

中文翻译：

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在体内评价与基质细胞衍生因子-1化学偶联的胶原膜的骨再生效果和异位成骨作用。

由于胶原蛋白膜缺乏骨诱导性，因此必须使用生物活性成分对其进行修饰以触发快速的骨再生。在这项研究中，我们旨在评估与基质细胞来源的因子-1α（SDF-1α）化学偶联的胶原膜的骨再生作用。为此，从四个组中选出了不同的胶原蛋白膜，包括具有Bio-Oss骨替代物+胶原蛋白膜的对照组。物理吸附基团，Bio-Oss +SDF-1α物理吸附在胶原膜上。Bio-Oss +SDF-1α与胶原膜化学交联的化学交联基团；并使用引导的骨再生技术将接种有Bio-Oss +骨髓间充质干细胞（BMSC）的细胞播种组置于临界尺寸的缺损模型中。在第4周和第8周，通过扫描电子显微镜，能量色散X射线光谱，微计算机断层扫描和组织形态分析对标本进行分析。此外，通过Von Kossa染色的组织学分析检查了异位成骨作用，并用苏木精和曙红和免疫组化染色对样品进行了染色。结果表明，与对照组和物理吸附组相比，化学交联组和细胞播种组的骨体积分数，骨表面积分数和小梁数目明显增加，并显示出更多的新骨形成。用苏木精和曙红复染的Von Kossa染色样品经过4周植入膜的免疫组织化学染色后发现，化学交联基团的微血管数量最多。与SDF-1α物理吸附相比，与SDF-1α化学偶联的胶原膜可显着促进新的骨骼和微血管形成，并且与BMSC播种方法相比，对新骨骼的形成具有相似的作用。这项研究提供了一种无细胞的方法来缩短骨骼的愈合时间并提高引导骨再生的成功率。