To decrease our dependency for the diminishing source of fossils resources, bio-based alternatives are being explored for the synthesis of commodity and high-value molecules. One example in this ecological initiative is the microbial production of the biosurfactant sophorolipids by the yeast Starmerella bombicola. Sophorolipids are surface-active molecules mainly used as household and laundry detergents. Because S. bombicola is able to produce high titers of sophorolipids, the yeast is also used to increase the portfolio of lipophilic compounds through strain engineering. Here, the one-step microbial production of hydroxy fatty acids by S. bombicola was accomplished by the selective blockage of three catabolic pathways through metabolic engineering. Successful production of 17.39 g/l (ω-1) linked hydroxy fatty acids was obtained by the successive blockage of the sophorolipid biosynthesis, the β-oxidation and the ω-oxidation pathways. Minor contamination of dicarboxylic acids and fatty aldehydes were successfully removed using flash chromatography. This way, S. bombicola was further expanded into a flexible production platform of economical relevant compounds in the chemical, food and cosmetic industries.

中文翻译：

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Starmerella bombicola生产长链羟基脂肪酸。

为了减少对化石资源来源的依赖，我们正在探索基于生物的替代品来合成商品和高价值分子。这一生态倡议中的一个例子是酵母小球菌Starmerella bombicola微生物生产生物表面活性剂槐糖脂。槐糖脂是表面活性分子，主要用作家用和洗衣粉。因为庞氏链球菌能够产生高滴度的槐糖脂，所以该酵母还被用于通过菌株工程来增加亲脂性化合物的种类。在这里，通过发酵工程选择性阻断三种分解代谢途径，可以实现单核糖链球菌一步一步生产羟基脂肪酸。成功生产17。通过连续阻断槐糖脂生物合成，β-氧化和ω-氧化途径，获得39 g / l（ω-1）连接的羟基脂肪酸。使用快速色谱法成功去除了二羧酸和脂肪醛的轻微污染。这样，S。bombicola进一步扩展到了化学，食品和化妆品行业中经济相关化合物的灵活生产平台。