Physeal injuries can lead to bony repair tissue formation, known as a bony bar. This can result in growth arrest or angular deformity, which is devastating for children who have not yet reached their full height. Current clinical treatment involves resecting the bony bar and replacing it with a fat graft to prevent further bone formation and growth disturbance, but these treatments frequently fail to do so and require additional interventions. Novel treatments that could prevent bone formation but also regenerate the injured physeal cartilage and restore normal bone elongation are warranted. To test the efficacy of these treatments, animal models that emulate human physeal injury are necessary. The rabbit model of physeal injury quickly establishes a bony bar, which can then be resected to test new treatments. Although numerous rabbit models have been reported, they vary in terms of size and location of the injury, tools used to create the injury, and methods to assess the repair tissue, making comparisons between studies difficult. The study presented here provides a detailed method to create a rabbit model of proximal tibia physeal injury using a two-stage procedure. The first procedure involves unilateral removal of 25% of the physis in a 6-week-old New Zealand white rabbit. This consistently leads to a bony bar, significant limb length discrepancy, and angular deformity within 3 weeks. The second surgical procedure involves bony bar resection and treatment. In this study, we tested the implantation of a fat graft and a photopolymerizable hydrogel as a proof of concept that injectable materials could be delivered into this type of injury. At 8 weeks post-treatment, we measured limb length, tibial angle, and performed imaging and histology of the repair tissue. By providing a detailed, easy to reproduce methodology to perform the physeal injury and test novel treatments after bony bar resection, comparisons between studies can be made and facilitate translation of promising therapies toward clinical use. Impact Statement This study provides details to create a rabbit model of physeal injury that can facilitate comparisons between studies and test novel regenerative medicine approaches. Furthermore, this model mimics the human, clinical situation that requires a bony bar resection followed by treatment. In addition, identification of a suitable treatment can be seen in the correction of the growth deformity, allowing this model to facilitate the development of novel physeal cartilage regenerative medicine approaches.

中文翻译：

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用于再生医学方法评估的家兔身体伤害模型。

身体受伤可导致骨修复组织形成，称为骨棒。这可能导致生长停滞或角畸形，这对于尚未达到其身高的孩子来说是毁灭性的。当前的临床治疗包括切除骨筋并用脂肪移植物替代骨筋，以防止进一步的骨形成和生长障碍，但是这些治疗常常无法做到，因此需要额外的干预措施。因此，有必要采取新颖的治疗方法来预防骨形成，但也可以使受伤的骨软骨再生并恢复正常的骨伸长。为了测试这些治疗方法的有效性，必须模拟人类血管损伤的动物模型。兔的骨干损伤模型很快建立了一根骨棒，然后可以将其切除以测试新的治疗方法。尽管已经报道了许多兔模型，但它们在损伤的大小和位置，造成损伤的工具以及评估修复组织的方法方面各不相同，因此很难进行研究之间的比较。本文介绍的研究提供了一种详细的方法，该方法可使用两步程序创建胫骨近端骨干近端损伤的兔子模型。第一个步骤包括单侧切除6周大的新西兰白兔体内25％的身体。在3周内，这始终会导致骨棒，明显的肢长差异和角畸形。第二种外科手术涉及骨棒切除和治疗。在这项研究中，我们测试了脂肪移植物和可光聚合水凝胶的植入，以证明可注射材料可用于此类伤害。治疗后8周，我们测量了肢体长度，胫骨角度，并对修复组织进行了成像和组织学检查。通过提供详细，易于复制的方法进行骨干切除术并测试骨棒切除后的新疗法，可以进行研究之间的比较，并有助于将有希望的疗法转化为临床应用。影响陈述这项研​​究提供了详细的信息，以创建兔子的骨eal损伤模型，该模型可以促进研究之间的比较和测试新的再生医学方法。此外，该模型模仿了需要进行骨棒切除并进行治疗的人类临床情况。此外，在矫正生长畸形时，可以找到合适的治疗方法，