We have showed that surface layer can determine cardiac effects of the magnetic nanoparticles (MNPs). Considering the high binding capacity of albumin and low side-effects, the aim of this study was to evaluate the influence of albumin coating on the cardiovascular effects of two manganese ferrite-based MNPs: citrate-coated and bare MNPs. Isolated rat hearts were perfused with citrate-coated magnetic nanoparticles (CiMNPs), citrate albumin-coated magnetic nanoparticles (CiAlbMNPs), bare magnetic nanoparticles (BaMNPs), and albumin-coated magnetic nanoparticles (AlbMNPs). CiMNPs induce a transient decrease in the left ventricular end-systolic pressure, +dP/dt and -dP/dt. These effects were not worsened by albumin coating. BaMNPs significantly increased the left ventricular end-diastolic pressure and perfusion pressure and decreased the +dP/dt and -dP/dt. These effects were completely absent in hearts perfused with AlbMNPs. None of the MNPs changed heart rate or arterial blood pressure in conscious rats. Magnetic signals in isolated hearts perfused with BaMNPs were significantly higher than AlbMNPs perfused hearts. However, the magnetic signal in heart tissue was similar when the MNPs were infused in conscious rats. These data indicate that albumin-coated can reduce cardiovascular effects of MNPs. These findings suggest a protective effect of albumin surface in MNPs, favoring its future therapeutic applications.

中文翻译：

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白蛋白涂层防止磁性纳米颗粒的心脏作用。

我们已经表明，表面层可以确定磁性纳米颗粒（MNP）的心脏效应。考虑到白蛋白的高结合能力和低副作用，本研究的目的是评估白蛋白涂层对两种基于锰铁氧体的MNPs（柠檬酸盐涂层的MNP和裸MNPs）的心血管作用的影响。用柠檬酸盐包覆的磁性纳米粒子（CiMNPs），柠檬酸盐包覆白蛋白的磁性纳米粒子（CiAlbMNPs），裸露磁性纳米粒子（BaMNPs）和白蛋白包覆的磁性纳米粒子（AlbMNPs）灌注离体的大鼠心脏。CiMNPs导致左心室收缩末压+ dP / dt和-dP / dt短暂降低。这些作用没有被白蛋白包衣所恶化。BaMNPs显着增加左心室舒张末期压力和灌注压力，并降低+ dP / dt和-dP / dt。在灌注AlbMNP的心脏中完全没有这些作用。MNP均未改变清醒大鼠的心率或动脉血压。灌注BaMNPs的离体心脏的磁信号明显高于AlbMNPs灌注的心脏。但是，在有意识的大鼠中注入MNP后，心脏组织中的磁信号相似。这些数据表明包被白蛋白可以降低MNP的心血管作用。这些发现表明白蛋白表面在MNP中具有保护作用，有利于其未来的治疗应用。灌注BaMNPs的离体心脏的磁信号明显高于AlbMNPs灌注的心脏。但是，在有意识的大鼠中注入MNP后，心脏组织中的磁信号相似。这些数据表明包被白蛋白可以降低MNP的心血管作用。这些发现表明白蛋白表面在MNP中具有保护作用，有利于其未来的治疗应用。灌注BaMNPs的离体心脏的磁信号明显高于AlbMNPs灌注的心脏。但是，在有意识的大鼠中注入MNP后，心脏组织中的磁信号相似。这些数据表明包被白蛋白可以降低MNP的心血管作用。这些发现表明白蛋白表面在MNP中具有保护作用，有利于其未来的治疗应用。