Deleterious effects from the freeze-thawing process on post-thawed sperm quality attributes is a main limiting factor in cryopreservation. The current study was conducted to determine the effect of semen extender containing zinc oxide (ZnO) on post-thaw rooster sperm quality indices.Semen samples from six, 60-week-old broiler breeder roosters were collected weekly during five successive weeks. The samples were mixed and divided to three equal parts and diluted with semen extender containing different levels of ZnO; 0 (ZnO-0), 1 or 2 (ZnO-2) µg/ml. After measuring thawing, motility and velocity parameters, plasma membrane functionality, apoptotic like changes, mitochondrial membrane potential (MMP), and DNA fragmentation index (DFI) were evaluated.Results showed that the addition of ZnO in extender quadratically affected (P<0.01) total motility (TM), progressive motility (PM), and average path velocity (VAP) with the highest values were noted in the ZnO-1 group. Levels of ZnO quadratically affected percentages of live (P<0.01), apoptotic (P<0.03) and dead (P<0.10) spermatozoa, where the highest percentage of live, and the lowest percentage of apoptotic or dead spermatozoa in the ZnO-1 group. Although adding ZnO quadratically affected plasma membrane functionality and MMP (P<0.01), it did not affect (P>0.05) DFI.In conclusion, there were some beneficial effects of ZnO supplementation in semen extender on post-thaw rooster sperm quality which may result in a better freezability.

中文翻译：

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氧化锌对公鸡精液冷冻保存的影响。

冻融过程对解冻后精子质量属性的有害影响是冷冻保存的主要限制因素。本研究旨在确定含氧化锌（ZnO）的精液补充剂对融化后公鸡精子质量指标的影响。连续五周每周收集六只，六十周大的肉鸡种鸡的精液样本。将样品混合并分成三等份，并用含有不同水平的ZnO的精液稀释剂稀释。0（ZnO-0），1或2（ZnO-2）µg / ml。在测量解冻，运动性和速度参数，质膜功能，凋亡样变化，线粒体膜电位（MMP）和DNA片段化指数（DFI）后，评估了增量剂中ZnO的添加受到二次影响（P <0。01）在ZnO-1组中记录了最高的总运动（TM），进行性运动（PM）和平均路径速度（VAP）。ZnO水平成倍地影响活（P <0.01），凋亡（P <0.03）和死亡（P <0.10）精子的百分比，其中ZnO-1中活的百分比最高，而凋亡或死亡的精子百分比最低组。尽管添加ZnO二次影响质膜功能和MMP（P <0.01），但不影响（P> 0.05）DFI。总之，在精液补充剂中添加ZnO对解冻后公鸡的精子质量有一些有益作用。导致更好的可冻结性。0.01），凋亡（P <0.03）和死亡（P <0.10）的精子，其中ZnO-1组中活的百分比最高，而凋亡或死亡的精子百分比最低。尽管添加ZnO二次影响质膜功能和MMP（P <0.01），但不影响（P> 0.05）DFI。总之，在精液补充剂中添加ZnO对解冻后公鸡的精子质量有一些有益作用。导致更好的可冻结性。0.01），凋亡（P <0.03）和死亡（P <0.10）的精子，其中ZnO-1组中活的百分比最高，而凋亡或死亡的精子百分比最低。尽管添加ZnO二次影响质膜功能和MMP（P <0.01），但不影响（P> 0.05）DFI。总之，在精液补充剂中添加ZnO对解冻后公鸡的精子质量有一些有益作用。导致更好的可冻结性。