Lumbar disc degeneration (LDD) is a common cause of low back and neck pain. The molecular mechanisms underlying LDD, however, are unclear. Noncoding RNAs have been reported to participate in human diseases. We investigated a series of public datasets (GSE67566, GSE56081 and GSE63492) and identified 568 mRNAs, 55 microRNAs (miRNAs), 765 long noncoding RNAs (lncRNAs), and 586 circular RNAs (circRNAs) that were expressed differently in LDD than in normal discs. We constructed lncRNAs and circRNAs regulated competing endogenous RNAs (ceRNA) networks in LDD. Four lncRNAs, DANCR, CASK-AS1, SCARNA2, and LINC00638), and three circRNAs, hsa_circ_0005139, hsa_circ_0037858, and hsa_circ_0087890, were identified as key regulators of LDD progression. We found that hsa-miR-486-5p regulated the crosstalk among circRNA hsa_circ_0000189, lncRNA DANCR and 6 mRNAs, PYCR2, TOB1, ARHGAP5, RBPJ, CD247, SLC34A1. Gene ontology (GO) analysis demonstrated that these differently expressed lncRNAs and circRNAs were involved in cellular component organization or biogenesis, gene expression and negative regulation of metabolic processes. Our findings provide useful information for exploring new mechanisms for LDD and candidates for therapeutic targets.

中文翻译：

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在人腰椎间盘退变中lncRNA和circRNA相关的ceRNA网络的系统鉴定。

腰椎间盘退变（LDD）是腰背和颈部疼痛的常见原因。然而，LDD的分子机制尚不清楚。据报道非编码RNA参与人类疾病。我们调查了一系列公共数据集（GSE67566，GSE56081和GSE63492），确定了568个mRNA，55个microRNA（miRNA），765个长非编码RNA（lncRNA）和586个环状RNA（circRNA），它们在LDD中的表达与正常光盘不同。 。我们在LDD中构建了lncRNA和circRNA调控竞争性内源性RNA（ceRNA）网络。四个lncRNA（DANCR，CASK-AS1，SCARNA2和LINC00638）和三个circRNA（hsa_circ_0005139，hsa_circ_0037858和hsa_circ_0087890）被确定为LDD进展的关键调控因子。我们发现hsa-miR-486-5p调节了circRNA hsa_circ_0000189，lncRNA DANCR和6个mRNA之间的串扰，PYCR2，TOB1，ARHGAP5，RBPJ，CD247，SLC34A1。基因本体论（GO）分析表明，这些不同表达的lncRNA和circRNA与细胞成分的组织或生物发生，基因表达和代谢过程的负调控有关。我们的发现为探索LDD的新机制和治疗靶标的候选者提供了有用的信息。