Fast 3D imaging and measurement of cells are of great importance in many fields associated with the life science. In this paper, only using two phase images in orthogonal directions, a geometric rotation method for 3D fast morphology reconstruction is proposed in view of more spheroid cells in blood cells. In this method, at first, the edges of a cell in two orthogonal projection directions are extracted from the corresponding phase images, and theirs centers and axes are determined. Then, with the geometric rotation method, the 3D surfaces of the cell membrane and its nuclei are reconstructed respectively. After all, the 3D complete morphology of the cell is reconstructed according to their space geometry relation. Simulation and experimental results demonstrate the validity and accuracy of this method for spheroid cells. Compared with other reconstruction methods, the image speed is improved since the multiple measurements or iterative procedures are not required. It provides a new approach to image the 3D morphological structure of spheroid blood cells.

细胞的快速3D成像和测量在与生命科学相关的许多领域中非常重要。在本文中，仅使用正交的两个相位图像，针对血细胞中更多的球状细胞，提出了一种用于3D快速形态重构的几何旋转方法。在该方法中，首先，从对应的相位图像中提取两个正交投影方向上的单元的边缘，并确定它们的中心和轴。然后，通过几何旋转方法，分别重建细胞膜及其细胞核的3D表面。毕竟，细胞的3D完整形态是根据它们的空间几何关系重建的。仿真和实验结果证明了该方法对球形细胞的有效性和准确性。与其他重建方法相比，由于不需要多次测量或迭代过程，因此提高了图像速度。它提供了一种新的方法来成像球状血细胞的3D形态结构。