Large-scale DNA sequencing, coupled with in silico gene trapping, is a robust approach to identifying unknown genes in selected genomic regions. Using this approach we have isolated a novel human gene, PROSC (for proline synthetase co-transcribed [bacterial homolog]), from human chromosome 8p11.2, and its mouse counterpart. The human PROSC gene spanned 17 kb of genomic DNA; its cDNA was 2530 bp long, with 8 exons that included an open reading frame of 825 bp (275 amino acids). The mouse cDNA (Prosc), 1995 bp long, was predicted to encode 274 amino acids. PROSC is ubiquitously expressed in human tissues and has been highly conserved among divergent species from bacteria to mammals, suggesting its important cellular function. The gene product is likely to be a soluble cytoplasmic protein, but its function remains to be determined.

中文翻译：

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人和小鼠PROSC（脯氨酸合成酶共转录）基因的克隆和表征。

大规模DNA测序与计算机基因捕获相结合，是一种在选定的基因组区域中鉴定未知基因的可靠方法。使用这种方法，我们从人染色体8p11.2及其小鼠对应物中分离出了新的人类基因PROSC（用于脯氨酸合成酶的共转录[细菌同源物]）。人类的PROSC基因跨越了17 kb的基因组DNA。它的cDNA长2530 bp，有8个外显子，其中包括825 bp（275个氨基酸）的开放阅读框。小鼠cDNA（Prosc）长1995 bp，预计可编码274个氨基酸。PROSC在人的组织中普遍表达，并且在从细菌到哺乳动物的各种物种中高度保守，表明其重要的细胞功能。基因产物可能是可溶性细胞质蛋白，但其功能尚待确定。