The main expression sites of HLA-G are human extravillous trophoblast cells. The interaction of HLA-G with uterine NK cells promotes their maturation and differentiation into decidual NK (dNK) cells. dNK cells secrete chemokines, cytokines, and proangiogenic factors in favor of a vascular remodeling and an immune suppressive microenvironment of the decidua. HLA-G is the most polymorphic member of the oligomorphic non-classical HLA molecule family; yet, the impact of polymorphic differences is not comprehensively understood. sHLA-G levels in embryo culture medium correlate with successful pregnancy; however, it remains questionable if HLA-G allelic diversity impacts on the outcome of dNK cell development. We utilized synthetic sHLA-G*01:01, 01:03, and 01:04 molecules and transduced K652/mHLA-G*01:01, 01:03, and 01:04 cells to study the biological interaction between HLA-G alleles and primary NK cells of human term placenta. Despite its low frequency, HLA-G*01:04 and not the most prevalent allele HLA-G*01:01 appear to be strong catalysts of dNK cell proliferation. Concluding, this study illustrates novel insights into the impact and binding efficiency of the three most common variants of HLA-G on primary placental NK cells.

中文翻译：

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胎盘中的微结构受HLA-G等位基因变体功能多样性的影响。

HLA-G的主要表达位点是人绒毛外滋养层细胞。HLA-G与子宫NK细胞的相互作用促进了它们的成熟和分化为蜕膜NK（dNK）细胞。dNK细胞分泌趋化因子，细胞因子和促血管生成因子，有利于蜕膜的血管重塑和免疫抑制性微环境。HLA-G是寡态非经典HLA分子家族中最多态的成员；但是，关于多态性差异的影响尚未得到全面了解。胚胎培养基中的sHLA-G水平与成功妊娠有关。但是，HLA-G等位基因多样性是否影响dNK细胞发育的结果仍然值得怀疑。我们利用合成的sHLA-G * 01：01、01：03和01:04分子并转导了K652 / mHLA-G * 01：01、01：03和01：04细胞用于研究HLA-G等位基因与人类足月胎盘原代NK细胞之间的生物学相互作用。尽管其频率较低，但HLA-G * 01：04而非最普遍的等位基因HLA-G * 01：01似乎是dNK细胞增殖的强大催化剂。最后，这项研究说明了HLA-G的三种最常见变体对原代胎盘NK细胞的影响和结合效率的新颖见解。