Bioinoculants are eco-friendly microorganisms, and their products are utilized for improving the potential of soil and fulfill the nutrients requirement for the host plant. The agricultural yield has increased due to the use of bioinoculants over chemical-based fertilizers, and thus it generates interest in understanding the innovation process by various methods. By gene-editing tool, the desired gene product can be changed for engineered microbial inoculants. We have also described various modern biotechnological tools like constraint-based modeling, OptKnock, flux balance analysis and modeling of the biological network for enhancing the bioinoculant capability. These fluxes give the fascinating perception of the metabolic network in the absence of comprehensive kinetic information. These tools also help in the stimulation of the metabolic networks by incorporation of enzyme-encoding genes. The present review explains the use of systems biology and gene-editing tools for improving the capability of bioinoculants. Moreover, this review also emphasizes on the challenges and future perspective of systems biology and its multidisciplinary facets.

中文翻译：

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通过代谢组学和系统生物学方法增强生物触媒能力。

生物杀菌剂是生态友好型微生物，其产品用于提高土壤潜力并满足宿主植物的养分需求。由于使用了生物灭活剂而不是化学肥料，农业产量增加了，因此引起了人们对通过各种方法了解创新过程的兴趣。通过基因编辑工具，可以为工程微生物接种剂改变所需的基因产物。我们还描述了各种现代生物技术工具，例如基于约束的建模，OptKnock，通量平衡分析和用于增强生物触媒剂能力的生物网络建模。在没有全面的动力学信息的情况下，这些通量给出了令人着迷的代谢网络感知。这些工具还通过掺入酶编码基因来帮助刺激代谢网络。本综述说明了使用系统生物学和基因编辑工具来提高生物抑制剂的能力。此外，这篇综述还着重于系统生物学及其多学科方面的挑战和未来展望。