Objectives: Lipid metabolism is closely associated with many important biological functions. Here, we conducted this study to explore the effects of gut microbiota on the lipid metabolism in the prefrontal cortex of mice. Methods: Germ-free (GF) mice, specific pathogen-free (SPF) and colonized GF (CGF) mice were used in this study. The open field test (OFT), forced swimming test (FST) and novelty suppressed feeding test (NSFT) were conducted to assess the changes in general behavioral activity. The liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) was used to obtain the lipid metabolites. Both one-way analysis of variance (one-way ANOVA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) were used to obtain the key differential lipid metabolites. Results: The behavioral tests showed that compared to SPF mice, GF mice had more center distance, more center time, less immobility time and less latency to familiar food. Meanwhile, 142 key differential lipid metabolites between SPF mice and GF mice were identified. These lipid metabolites mainly belonged to glycerophospholipids, glycerolipids, sphingolipids, and saccharolipids. The gut microbiota colonization did not reverse these changed behavioral phenotypes, but could restore 25 key differential lipid metabolites. Discussion: These results showed that the absence of gut microbiota could influence host behaviors and lipid metabolism. Our findings could provide original and valuable data for future studies to further investigate the microbiota-gut-brain axis.

中文翻译：

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肠道菌群的缺乏会影响小鼠前额叶皮层的脂质代谢。

目的：脂质代谢与许多重要的生物学功能密切相关。在这里，我们进行了这项研究，以探索肠道菌群对小鼠前额叶皮层脂质代谢的影响。方法：本研究使用无病菌（GF）小鼠，无特定病原体（SPF）和定植的GF（CGF）小鼠。进行了野外试验（OFT），强迫游泳试验（FST）和新奇抑制进食试验（NSFT），以评估一般行为活动的变化。液相色谱-串联质谱法（LC-MS / MS）用于获得脂质代谢物。方差的单向分析（单向方差分析）和正交偏最小二乘判别分析（OPLS-DA）均用于获得关键的脂质代谢产物。结果：行为测试表明，与SPF小鼠相比，GF小鼠具有更大的中心距离，更多的中心时间，更少的固定时间以及更少的熟悉食物的等待时间。同时，在SPF小鼠和GF小鼠之间鉴定了142个关键的脂质代谢产物。这些脂质代谢物主要属于甘油磷脂，甘油脂，鞘脂和糖脂。肠道菌群定殖不能逆转这些改变的行为表型，但可以恢复25种关键的脂质代谢产物。讨论：这些结果表明，肠道菌群的缺乏会影响宿主行为和脂质代谢。我们的发现可以为进一步研究微生物群-肠脑轴的未来研究提供原始和有价值的数据。鉴定了SPF小鼠和GF小鼠之间的142个关键脂质代谢产物。这些脂质代谢物主要属于甘油磷脂，甘油脂，鞘脂和糖脂。肠道菌群定殖不能逆转这些改变的行为表型，但可以恢复25种关键的脂质代谢产物。讨论：这些结果表明，肠道菌群的缺乏会影响宿主行为和脂质代谢。我们的发现可以为进一步研究微生物群-肠脑轴的未来研究提供原始和有价值的数据。鉴定了SPF小鼠和GF小鼠之间的142个关键脂质代谢产物。这些脂质代谢物主要属于甘油磷脂，甘油脂，鞘脂和糖脂。肠道菌群定殖不能逆转这些改变的行为表型，但可以恢复25种关键的脂质代谢产物。讨论：这些结果表明，肠道菌群的缺乏会影响宿主行为和脂质代谢。我们的发现可以为进一步研究微生物群-肠脑轴的未来研究提供原始和有价值的数据。但可以恢复25种关键的脂质代谢产物。讨论：这些结果表明，肠道菌群的缺乏会影响宿主行为和脂质代谢。我们的发现可以为进一步研究微生物群-肠脑轴的未来研究提供原始和有价值的数据。但可以恢复25种关键的脂质代谢产物。讨论：这些结果表明，肠道菌群的缺乏会影响宿主行为和脂质代谢。我们的发现可以为进一步研究微生物群-肠脑轴的未来研究提供原始和有价值的数据。