Several species of the Annonaceae plants have been used as complementary medicine for cancer-associated illnesses in some ethnic groups of northern Thailand. This study investigated the cytotoxic and cytostatic activity of methanolic extracts derived from the stems of these plants, including Uvaria longipes (Craib) L.L.Zhou, Y.C.F.Su & R.M.K.Saunders, Artabotrys burmanicus A.DC, Marsypopetalum modestum (Pierre) B.Xue & R.M.K.Saunders, and Dasymaschalon sp. Cell death induction of seven human cancer cell lines and cell cycle analyses were assessed by Annexin V and/or propidium iodide (PI) staining and analyzed by flow cytometry. Treatment of cancer cell lines with the extract of four Annonaceae plants resulted in various cytotoxic activities depending on cell type. The extract of U. longipes exhibited the highest cytotoxic activity capable of inducing cell death of several cancer cell lines, particularly against hepatocellular carcinoma cell lines (HepG2 and Hep3B). This extract was capable of inducing cell cycle arrest at the SubG1 phase. Phytochemical screening of all the extracts revealed the presence of alkaloids, sterols, tannins, anthraquinone glycoside, coumarin, and flavonoids. Determination of active compounds by high-performance liquid chromatography standards revealed bullatacin and asiminecin in all the extracts. The extract of Annonaceae stem or its compounds may provide an opportunity for the development of new therapies against cancer.

中文翻译：

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四种番荔枝科植物对人癌细胞系的细胞毒性和细胞抑制作用。

在泰国北部的一些民族中，几种番荔枝科植物已被用作癌症相关疾病的补充药物。这项研究调查了从这些植物的茎中提取的甲醇提取物的细胞毒性和细胞抑制活性，这些植物包括长叶乌贼（Craib）LLZhou，YCFSu和RMKSaunders，Artabotrys burmanicus A.DC，Marsypopetalum Modetum（Pierre）B.Xue＆RMKSaunders和Dasymaschalon sp。通过膜联蛋白V和/或碘化丙啶（PI）染色评估了7种人类癌细胞系的细胞死亡诱导和细胞周期分析，并通过流式细胞仪进行了分析。用四种番荔枝科植物的提取物处理癌细胞系会导致不同的细胞毒活性，具体取决于细胞类型。U的提取物 长瓣虫具有最高的细胞毒性活性，能够诱导几种癌细胞系的细胞死亡，尤其是针对肝细胞癌细胞系（HepG2和Hep3B）。该提取物能够诱导SubG1期细胞周期停滞。对所有提取物进行植物化学筛选后，发现存在生物碱，固醇，单宁，蒽醌糖苷，香豆素和类黄酮。通过高效液相色谱标准物测定活性化合物，结果发现所有提取物中均含有布洛他星和阿斯米星。番荔枝科茎或其化合物的提取物可能为开发抗癌新疗法提供机会。该提取物能够诱导SubG1期细胞周期停滞。对所有提取物进行植物化学筛选后，发现存在生物碱，固醇，单宁，蒽醌糖苷，香豆素和类黄酮。通过高效液相色谱标准物测定活性化合物，结果发现所有提取物中均含有布洛他星和阿斯米星。番荔枝科茎或其化合物的提取物可能为开发抗癌新疗法提供机会。该提取物能够诱导SubG1期细胞周期停滞。对所有提取物进行植物化学筛选后，发现存在生物碱，固醇，单宁，蒽醌糖苷，香豆素和类黄酮。通过高效液相色谱标准物测定活性化合物，结果发现所有提取物中均含有布洛他星和阿斯米星。番荔枝科茎或其化合物的提取物可能为开发抗癌新疗法提供机会。