Abstract Although effective, the embedment of bone tissue ancl orthopaeclic devices using polymethylmethacrylate (PMMA) has challenges and limitations. To embed using PMMA. specimens must first be fixed in 70% ethanol, dehydrated in ascending grades of ethanol, and then infiltrated ancl polymerized in polymethylmethacrylate using standard techniques. This process can take more than 22 d for large bone specimens. Additionally, PMMA embedment has been shown to dissolve bone cement. thus enabling the analysis of the bone–cement interfaces. To conserve processing time while preserving the bone-cement interface, a method was developed for processing mineralized bone tissue in preparation for scanning electron microscopy (SEM) imaging that does not require PMMA embedment. This technique does not require the traditional dehydration and PMMA polymerization process. Instead. fresh mineralized cemented bone specimens were serially sectioned and the marrow removed after formalin fixation. The sections were air-dried then desiccated. The sections were then prepared for SEM imaging and examination. This process takes a fraction of the tissue processing time while not compromising the bone-cement integrity. The SEM image quality was shown to be comparative to images obtained with PMMA-embedded bone specimens. (The J Histotecllriol 29:229. 2006) Submitted August 14, 2006; accepted with revisions October 31, 2006

中文翻译：

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一种无聚甲基丙烯酸甲酯包埋的骨水泥界面检查方法

摘要 虽然有效，但使用聚甲基丙烯酸甲酯 (PMMA) 嵌入骨组织和骨科装置具有挑战和局限性。使用 PMMA 嵌入。标本必须首先在 70% 乙醇中固定，在升序乙醇中脱水，然后使用标准技术渗透在聚甲基丙烯酸甲酯中聚合的 ancl。对于大型骨骼标本，此过程可能需要 22 天以上。此外，PMMA 嵌入已被证明可以溶解骨水泥。从而能够分析骨水泥界面。为了在保留骨水泥界面的同时节省处理时间，开发了一种处理矿化骨组织的方法，为不需要 PMMA 嵌入的扫描电子显微镜 (SEM) 成像做准备。该技术不需要传统的脱水和 PMMA 聚合过程。反而。新鲜矿化骨水泥连续切片，福尔马林固定后取出骨髓。将切片风干然后干燥。然后准备切片用于 SEM 成像和检查。这个过程只需要一小部分组织处理时间，同时不会影响骨水泥的完整性。SEM 图像质量与使用 PMMA 嵌入的骨标本获得的图像相当。(The J Histotecllriol 29:229. 2006) 2006 年 8 月 14 日提交；2006 年 10 月 31 日修订后接受 然后准备切片用于 SEM 成像和检查。这个过程只需要一小部分组织处理时间，同时不会影响骨水泥的完整性。SEM 图像质量与使用 PMMA 嵌入的骨标本获得的图像相当。(The J Histotecllriol 29:229. 2006) 2006 年 8 月 14 日提交；2006 年 10 月 31 日修订后接受 然后准备切片用于 SEM 成像和检查。这个过程只需要一小部分组织处理时间，同时不会影响骨水泥的完整性。SEM 图像质量与使用 PMMA 嵌入的骨标本获得的图像相当。(The J Histotecllriol 29:229. 2006) 2006 年 8 月 14 日提交；2006 年 10 月 31 日修订后接受