The mouse embryo hindbrain is a robust and adaptable model for studying sprouting angiogenesis. It permits the spatiotemporal analysis of organ vascularization in normal mice and in mouse strains with genetic mutations that result in late embryonic or perinatal lethality. Unlike postnatal models such as retinal angiogenesis or Matrigel implants, there is no requirement for the breeding of conditional knockout mice. The unique architecture of the hindbrain vasculature allows whole-mount immunolabeling of blood vessels and high-resolution imaging, as well as easy quantification of angiogenic sprouting, network density and vessel caliber. The hindbrain model also permits the visualization of ligand binding to blood vessels in situ and the analysis of blood vessel growth within a natural multicellular microenvironment in which endothelial cells (ECs) interact with non-ECs to refine the 3D organ architecture. The entire procedure, from embryo isolation to imaging and through to results analysis, takes approximately 4 d.

中文翻译：

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胚胎小鼠后脑作为研究血管生成的分子和细胞机制的定性和定量模型。

小鼠胚胎后脑是研究发芽血管生成的强大且适应性强的模型。它允许对正常小鼠和具有导致晚期胚胎或围产期致死率的基因突变的小鼠品系的器官血管化进行时空分析。与视网膜血管生成或基质胶植入物等产后模型不同，不需要条件性基因敲除小鼠的繁殖。后脑脉管系统的独特结构允许对血管进行整体免疫标记和高分辨率成像，以及轻松量化血管生成发芽、网络密度和血管口径。后脑模型还允许在原位观察配体与血管的结合，并分析自然多细胞微环境中的血管生长，其中内皮细胞 (EC) 与非 EC 相互作用以改进 3D 器官结构。整个过程，从胚胎分离到成像，再到结果分析，大约需要 4 天。