The ability to combine a selective capture strategy with on chip MALDI-TOF analysis allows for rapid, sensitive analysis of a variety of different analytes. In this overview a series of applications of capture enhanced laser desorption ionization time of flight (CELDI-TOF) mass spectrometry are described. The key feature of the assay is an off-chip capture step that utilizes high affinity bacterial binding proteins to capture a selected ligand. This allows large volumes of sample to be used and provides for a concentration step prior to transfer to a gold chip for traditional mass spectral analysis. The approach can also be adapted to utilize specific antibody as the basis of the capture step. The direct and indirect CELDI-TOF assays are rapid, reproducible and can be a valuable proteomic tool for analysis of low abundance molecules present in complex mixtures like blood plasma.

中文翻译：

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用于复杂生物样品质谱分析的新型样品制备

将选择性捕获策略与片上 MALDI-TOF 分析相结合的能力允许对各种不同的分析物进行快速、灵敏的分析。在本概述中，描述了捕获增强型激光解吸电离飞行时间 (CELDI-TOF) 质谱的一系列应用。该测定的关键特征是芯片外捕获步骤，它利用高亲和力细菌结合蛋白来捕获选定的配体。这允许使用大量样品并在转移到用于传统质谱分析的金芯片之前提供浓缩步骤。该方法还可以适用于利用特定抗体作为捕获步骤的基础。直接和间接 CELDI-TOF 检测快速、