Bioconjugation is a burgeoning field of research. Novel methods for the mild and site-specific derivatization of proteins, DNA, RNA, and carbohydrates have been developed for applications such as ligand discovery, disease diagnosis, and high-throughput screening. These powerful methods owe their existence to the discovery of chemoselective reactions that enable bioconjugation under physiological conditions-a tremendous achievement of modern organic chemistry. Here, we review recent advances in bioconjugation chemistry. Additionally, we discuss the stability of bioconjugation linkages-an important but often overlooked aspect of the field. We anticipate that this information will help investigators choose optimal linkages for their applications. Moreover, we hope that the noted limitations of existing bioconjugation methods will provide inspiration to modern organic chemists.

中文翻译：

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生物共轭的进展


生物共轭是一个新兴的研究领域。蛋白质、DNA、RNA 和碳水化合物的温和和位点特异性衍生化的新方法已经开发出来，用于配体发现、疾病诊断和高通量筛选等应用。这些强大的方法的存在归功于化学选择性反应的发现，这些化学选择性反应能够在生理条件下实现生物共轭，这是现代有机化学的巨大成就。在这里，我们回顾生物共轭化学的最新进展。此外，我们还讨论了生物共轭连接的稳定性——该领域的一个重要但经常被忽视的方面。我们预计这些信息将帮助研究人员为其应用选择最佳连接。此外，我们希望现有生物共轭方法的局限性能为现代有机化学家提供灵感。