We tested that hypothesis that slow re-warming rates would improve the ability of Drosophila melanogaster Meigen larvae to survive acute low temperature exposure. Four larval stages (1(st), 2(nd), 3(rd) instars and wandering stage 3(rd) instars) of four wild-type strains were exposed to -7 degrees C for periods of time expected to result in 90 % mortality. Larvae were then either directly transferred to their rearing temperature (21 degrees C), or returned to this temperature in a stepwise fashion (pausing at 0 and 15 degrees C) or by slow warming at 1 or 0.1 degrees C/min. We observed a reduced rapid cold-hardening effect and no general increase in survival of acute chilling in larvae re-warmed in a stepwise or slow fashion, and hypothesise that slow re-warming may result in accumulation of further chill injuries.

中文翻译：

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急性低温暴露后缓慢和阶梯式复温不会提高黑腹果蝇幼虫的存活率。

我们测试了这种假设，即缓慢的复温率会提高黑腹果蝇幼虫在急性低温暴露中存活的能力。四个野生型菌株的四个幼虫阶段（1(st)、2(nd)、3(rd) 龄和游荡阶段 3(rd) 龄）暴露于 -7 摄氏度的时间，预计将导致 90 % 死亡率。然后将幼虫直接转移到它们的饲养温度（21 摄氏度），或以逐步方式（在 0 和 15 摄氏度暂停）或以 1 或 0.1 摄氏度/分钟的速度缓慢升温返回到该温度。我们观察到在以逐步或缓慢方式重新加热的幼虫中，快速冷硬化效应降低，并且急性寒冷的存活率没有普遍增加，并且假设缓慢重新加热可能导致进一步的寒冷伤害积累。