The mouse Dlx5 gene encodes a distal-less-related DNA-binding homeobox protein first expressed during early embryonic development in anterior regions of mouse embryo and is located on chromosome 6, which is the syntenic region to the human chromosome 7q21-q31 imprinting cluster. Recently, its human homologue, DLX5, was identified to be imprinted and maternally expressed, at least in normal human lymphoblasts and in brain tissues. In our study, we analyzed the imprinting status of mouse Dlx5 by RT-PCR, first in the F1 of a reciprocal cross between two different mouse strains, and second in heterozygous Dlx5 mutant mice. Both approaches revealed that mouse Dlx5 followed a biallelic pattern of expression in brain tissue and in testis. Our findings suggest that the Dlx5 gene escapes genomic imprinting, at least in mice of certain genetic backgrounds.

中文翻译：

---

Dlx5是人类印迹的DLX5基因的小鼠同源物，在小鼠大脑中双等位表达。

小鼠Dlx5基因编码与远端无关的DNA结合同源异型盒蛋白，该蛋白首先在早期胚胎发育过程中在小鼠胚胎的前部区域表达，并位于第6号染色体上，该染色体是人类7q21-q31染色体印迹簇的同位区域。最近，至少在正常的人类淋巴母细胞和脑组织中，其人类同源物DLX5被鉴定为印记并在母亲中表达。在我们的研究中，我们通过RT-PCR分析了小鼠Dlx5的印迹状态，首先是在两种不同小鼠品系之间相互杂交的F1中，其次是在杂合性Dlx5突变小鼠中。两种方法都表明小鼠Dlx5在脑组织和睾丸中遵循双等位基因表达模式。我们的发现表明，Dlx5基因至少在某些遗传背景的小鼠中逃脱了基因组印迹。