To examine temporal changes in a bacterial community, water samples were collected monthly for one year from five sites along a major use-reuse river, the Cuyahoga River, in northeastern Ohio (USA). Fluorescent in situ hybridization (FISH) was used to enumerate population sizes of two species of common bacteria, Pseudomonas putida and Acinetobacter calcoaceticus; FISH was also performed with a Domain Bacteria specific probe. In addition, the total bacteria (based on DAPI staining), colony forming units (on modified Nutrient agar) and coliforms were enumerated and supporting physical/chemical data were collected. Each variable examined exhibited a different seasonal pattern. Temporal changes in total number of bacteria and population size of P. putida were correlated with turbidity and precipitation suggesting that allochthonous sources and scouring of the benthos may be major contributors to these portions of the community. In contrast, the number of cells hybridizing the Domain Bacteria and A. calcoaceticus probes were correlated with temperature. Thus, different aspects of the bacterial community are potentially controlled by different factors and the role of allochthonous and autochthonous sources may vary among species.

中文翻译：

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东北俄亥俄河（美国）浮游细菌的时间变化。

为了检查细菌群落的时间变化，我们从俄亥俄州东北部的一条主要重用河流Cuyahoga河（美国）沿途的五个地点每月收集了一年的水样。荧光原位杂交（FISH）用于计算两种常见细菌恶臭假单胞菌和钙乙酸不动杆菌的种群大小。还使用域细菌特异性探针进行了FISH。此外，列举了总细菌（基于DAPI染色），菌落形成单位（在改良的营养琼脂上）和大肠菌群，并收集了支持的理化数据。检验的每个变量都表现出不同的季节性模式。细菌总数和种群数量随时间变化 恶臭与腐浊度和降水量相关，表明异源和底栖生物的冲刷可能是社区中这些部分的主要来源。相反，与域细菌和钙乙酸拟南芥探针杂交的细胞数量与温度相关。因此，细菌群落的不同方面可能受到不同因素的控制，不同物种中异源和本地来源的作用可能有所不同。