When cells are first exposed to low levels of oxidative stress, they develop a resistance to a subsequent challenge of the same stress, even at higher levels. Although some protein(s) induced by oxidative stress likely mediated this adaptive response, the nature of these proteins is unknown. In this study, the total proteins extracted from human U937 leukemia cells exposed to 50 micromM H(2)O(2) for 24 h to induce an optimal protective response were analyzed by two-dimensional polyacrylamide gel electrophoresis. H(2)O(2) treatment induced elevation of level of 34 protein spots. An analysis of these spots by a matrix associated laser desorption/ionization time-of-flight mass spectrometry identified 28 of the H(2)O(2)-induced proteins. These include proteins involved in energy metabolism, translation and RNA processing, chaperoning or mediating protein folding, cellular signaling, and redox regulation, as well as a mitochondrial channel component, and an actin-bundling protein. Therefore, it appears that the cellular adaptation to oxidative stress is a complex process, and is accompanied by a modulation of diverse cellular functions.

中文翻译：

---

人U937细胞中适应浓度的过氧化氢诱导的细胞蛋白的蛋白质组学分析。

当细胞最初暴露于低水平的氧化应激时，即使在较高水平下，它们也会对随后的相同压力产生抵抗力。尽管由氧化应激诱导的某些蛋白质可能介导了这种适应性反应，但这些蛋白质的性质尚不清楚。在这项研究中，通过二维聚丙烯酰胺凝胶电泳分析了从人类U937白血病细胞中暴露于50 micromM H（2）O（2）24小时以诱导最佳保护反应的总蛋白。H（2）O（2）处理诱导34个蛋白质斑点的水平升高。通过矩阵相关的激光解吸/电离飞行时间质谱对这些斑点的分析确定了H（2）O（2）诱导蛋白中的28个。其中包括参与能量代谢，翻译和RNA加工的蛋白质，伴侣或介导的蛋白质折叠，细胞信号传导和氧化还原调节，以及线粒体通道成分和肌动蛋白捆绑蛋白。因此，看来细胞对氧化应激的适应是一个复杂的过程，并伴随着多种细胞功能的调节。