The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has proven to be a powerful tool in plant genetic transformation studies. This paper reviews the history and the progression of the expression of GFP variants in transgenic plants. The distinguishing features of the most useful GFPs, such as those including the S65T chromophore mutation and those with dual excitation peaks, are discussed. The review also focuses on the utility of GFP as a visual selectable marker in aiding the plant transformation process; GFP has been more important in monocot transformation compared with dicot transformation. Finally, the potential utility of new fluorescent proteins is speculated upon.

中文翻译：

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绿色荧光蛋白在转基因植物中的用途。

事实证明，来自水母维多利亚水母的绿色荧光蛋白（GFP）是植物遗传转化研究中的有力工具。本文回顾了转基因植物中GFP变体表达的历史和进展。讨论了最有用的GFP的区别特征，例如包括S65T发色团突变和具有双重激发峰的GFP。这篇综述还着重介绍了GFP在帮助植物转化过程中作为视觉选择标记的用途。与双子叶植物转化相比，GFP在单子叶植物转化中更为重要。最后，推测了新型荧光蛋白的潜在用途。