A simple counter-staining procedure has been developed for comparative beta-glucuronidase (GUS) expression and anatomical localization in transgenic herbaceous arabidopsis and tobacco. This protocol provides good anatomical visualization for monitoring chimeric gene expression at both the organ and tissue levels. It can be used with different histochemical stains and can be extended to the study of woody species. The specimens are paraffin-embedded, the block is trimmed to reveal internal structure, safranin-O staining solution is briefly applied to the surface of the block, then washed off and, after drying, a drop of immersion oil is placed on the stained surface for subsequent photographic work. This gives tissue counter-staining with good structural preservation without loss of GUS staining product; moreover, sample observation is rapid and efficient compared to existing procedures.

中文翻译：

---

用于转基因拟南芥和烟草中 GUS 表达定位的样本块复染。


已经开发出一种简单的复染程序，用于比较转基因草本拟南芥和烟草中的 β-葡萄糖醛酸酶 (GUS) 表达和解剖定位。该协议为监测器官和组织水平的嵌合基因表达提供了良好的解剖可视化。它可以与不同的组织化学染色剂一起使用，并且可以扩展到木本物种的研究。将标本石蜡包埋，修整块以显示内部结构，将番红-O染色溶液短暂涂在块的表面，然后洗掉，干燥后，将一滴浸油放在染色的表面上以便后续的摄影工作。这使得组织复染具有良好的结构保存，而不会损失 GUS 染色产物；此外，与现有程序相比，样本观察快速高效。