Inducible promoters or gene-switches are used to both spatially and temporally regulate gene expression. Such regulation can provide information concerning the function of a gene in a developmental context as well as avoid potential harmful effects due to overexpression. A gfp construct under the control of a copper-inducible promoter was introduced into Arabidopsis thaliana (L.) Heynh. and the regulatory parameters of this inducible promoter were determined. Here, we describe the time-course of up- and down-regulation of GFP expression in response to copper level, the optimal regulatory levels of copper, and the tissue specificity of expression in three transgenic lines. We conclude that the copper-inducible promoter system may be useful in regulating the time and location of gene expression in A. thaliana.

中文翻译：

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拟南芥中酵母铜诱导的启动子系统的表征。

诱导型启动子或基因开关用于在空间和时间上调节基因表达。这种调节可以提供有关基因在发育中的功能的信息，并避免由于过表达而引起的潜在有害作用。将在铜诱导型启动子控制下的gfp构建体引入拟南芥（L.）Heynh。确定了该诱导型启动子的调控参数。在这里，我们描述了响应于铜水平，铜的最佳调节水平以及在三个转基因品系中表达的组织特异性，GFP表达上调和下调的时间过程。我们得出结论，铜诱导的启动子系统可能在调节拟南芥中基因表达的时间和位置中有用。