The aniline dye industry was created in 1856 when the Englishman William Perkin prepared the color mauve from aniline. Dye industries were then formed in several European countries, and each year thousands of new colors and shades were tested on fabrics. Scientists of that period used these new dyes to study the structures of plant and animal tissues. Starting in 1880 George Grubler, a German pharmacist, sold biological stains to scientists around the world. Grubler stains earned a reputation for reliability, and many of the staining methods, developed at that time stiuplated the use of Grubler's dyes, even after the firm was sold to Karl Hollborn who continued Grubler's work and devised new staining methods. The Grubler company continued to prosper until World War I. The Chroma Gesellschaft-Schmid GMBH Company in Germany is a successor of the Grubler company. The interrupted supply of Grubler stains during World War I prompted the start of the American biological stain industry and subsequently the Biological Stain Commission was formed.

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George Grubler 和 Karl Hollborn：生物染色行业的两位创始人

苯胺染料工业诞生于 1856 年，当时英国人 William Perkin 用苯胺制备了紫红色。然后在几个欧洲国家形成了染料工业，每年都有数千种新颜色和色调在织物上进行测试。那个时期的科学家使用这些新染料来研究植物和动物组织的结构。从 1880 年开始，德国药剂师 George Grubler 向世界各地的科学家出售生物染色剂。Grubler 染色剂赢得了可靠性的声誉，当时开发的许多染色方法刺激了 Grubler 染料的使用，即使在该公司被出售给继续 Grubler 的工作并设计新染色方法的 Karl Hollborn 之后也是如此。Grubler 公司一直繁荣到第一次世界大战。德国的 Chroma Gesellschaft-Schmid GMBH 公司是 Grubler 公司的继承者。第一次世界大战期间格鲁勒染色剂供应中断促使美国生物染色剂行业的开始，随后成立了生物染色剂委员会。