The carbon kinetic isotope effects associated with synthesis of acetate from CO2 and H2 during autotrophic growth of Acetobacterium woodii at 30 degrees C have been measured by isotopic analyses of CO2, methyl-carbon, and total acetate. Closed systems allowing construction of complete mass balances at varying stages of growth were utilized, and the effects of the partitioning of carbon between CO2 and HCO3- were taken account. For the overall reaction, total carbonate --> total acetate, isotope effects measured in replicate experiments ranged from -59.0 +/- 0.9% to -57.2 +/- 2.3%. Taking into account all measurements, the weighted mean and standard deviation are -58.6 +/- 0.7%. There is no evidence for intramolecular ordering in the acetate. The carbon isotopic composition of sedimentary acetate, otherwise expected to be near that of sedimentary organic carbon, is likely to be depleted in environments in which autotrophic acetogenesis is occurring.

中文翻译：

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与自养产乙酸相关的碳同位素效应

碳动力学同位素效应与从 CO2 和 H2 合成醋酸盐在 30 摄氏度的木醋杆菌自养生长过程中相关联，已通过 CO2、甲基碳和总醋酸盐的同位素分析进行​​了测量。封闭系统允许在不同的生长阶段构建完整的质量平衡，并考虑了碳在 CO2 和 HCO3- 之间分配的影响。对于整个反应，总碳酸盐 --> 总乙酸盐，在重复实验中测得的同位素效应范围从 -59.0 +/- 0.9% 到 -57.2 +/- 2.3%。考虑到所有测量值，加权平均值和标准偏差为 -58.6 +/- 0.7%。没有证据表明醋酸盐分子内有序。沉积醋酸盐的碳同位素组成，