A piezoelectric crystal immunosensor has been developed for the detection and determination of cortisol. Cortisol antibody was layered onto the gold electrodes of a 10 MHz piezoelectric crystal which was pre-coated with either protein A or gluteraldehyde. Crystals pre-coated with protein A showed the best results with respect to stability and sensitivity. The sensor was successfully used for the determination of cortisol in standard solutions from 36-3628 micrograms/L (part per billion). The advantages of the proposed sensor include simplicity, short analysis time, cost effectiveness and selectivity. The results demonstrate the feasibility of cortisol assay in clinical testing and in drug monitoring.

中文翻译：

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用于检测皮质醇的压电免疫传感器

压电晶体免疫传感器已被开发用于检测和测定皮质醇。皮质醇抗体层叠在 10 MHz 压电晶体的金电极上，该晶体预涂有蛋白 A 或戊二醛。用蛋白质 A 预包被的晶体在稳定性和灵敏度方面表现出最好的结果。该传感器已成功用于测定标准溶液中 36-3628 微克/升（十亿分之一）的皮质醇。所提出的传感器的优点包括简单、分析时间短、成本效益和选择性。结果证明了皮质醇测定在临床测试和药物监测中的可行性。