Gas trapped in the interstices of the biomaterials used for vascular prostheses causes thrombosis, and the process of eliminating this gas is known as denucleation. An apparatus was developed for testing the in vitro effects of denucleation on 4 mm I.D. expanded polytetrafluoroethylene (ePTFE) Vitagraft (Johnson and Johnson). The apparatus was designed to ensure that neither the blood nor the grafts came in contact with air. Blood from a single donor was incubated with control and denucleated grafts for 5, 10, 15, 20, and 30 minutes. The thrombus volume in the graft lumen was measured with a computer assisted videometric system. Little thrombus formed by 5 or 10 minutes, but there was less thrombus in the denucleated graft than in the con trol graft at all times. The differences were statistically significant at 15 and 20 minutes (p < 0.05). Denucleation nearly doubled the thrombus formation time. Thrombus was more adherent to denucleated grafts than to control grafts. These results are consistent with in vivo observations in the rat where denucleation decreased thrombus formation and increased patency duration.

中文翻译：

---

间隙空气对 ePTFE 血管移植物体外血栓形成的影响

被困在用于血管假体的生物材料的空隙中的气体会导致血栓形成，消除这种气体的过程称为去核。开发了一种装置，用于测试去核对 4 mm ID 膨胀聚四氟乙烯 (ePTFE) Vitaraft (Johnson and Johnson) 的体外影响。该装置旨在确保血液和移植物均不与空气接触。来自单个供体的血液与对照和去核移植物孵育 5、10、15、20 和 30 分钟。移植管腔中的血栓体积用计算机辅助视频测量系统测量。5 或 10 分钟后形成的血栓很少，但在任何时候，去核移植物中的血栓都少于对照移植物中的血栓。差异在 15 和 20 分钟时具有统计学意义（p < 0.05）。去核使血栓形成时间几乎加倍。与对照移植物相比，血栓对去核移植物的粘附性更强。这些结果与大鼠体内观察一致，其中去核减少血栓形成并增加通畅持续时间。