Our previous screening of a Glossina morsitans morsitans lamdagt11 salivary gland expression library with serum of a tsetse fly exposed rabbit identified a cDNA encoding Tsetse Antigen5 (TAg5, 28.9 kDa), a homologue of Antigen5 sting venom allergens. Recombinant TAg5 was produced in Sf9 cells in order to assess its immunogenic properties in humans. Plasma from a patient that previously exhibited anaphylactic reactions against tsetse fly bites contained circulating anti-TAg5 and anti-saliva IgEs. In a significant proportion of plasma samples of African individuals, TAg5 and saliva binding IgEs (respectively 56 and 65%) can be detected. Saliva, harvested from flies that were subjected to TAg5- specific RNA interference (RNAi), displayed significantly reduced IgE binding potential. Allergenic properties of TAg5 and tsetse fly saliva were further illustrated in immunized mice, using an immediate cutaneous hypersensitivity and passive cutaneous anaphylaxis assay. Collectively, TAg5 was illustrated to be a tsetse fly salivary allergen, demonstrating that Antigen5-related proteins are represented as functional allergens not only in stinging but also in blood feeding insects.

中文翻译：

---

在采血昆虫（采采蝇）的唾液中鉴定出功能性Antigen5相关过敏原。

我们以前用采采蝇蝇暴露的兔子的血清筛选了Glossina morsitans morsitans lamdagt11唾液腺表达文库，确定了一个编码Tsetse Antigen5（TAg5，28.9 kDa）的cDNA，它是Antigen5 st毒过敏原的同源物。为了评估其在人类中的免疫原性，在Sf9细胞中产生了重组TAg5。先前对采采蝇蝇叮咬有过敏反应的患者血浆中含有循环的抗TAg5和抗唾液IgE。在非洲人血浆样本的很大一部分中，可以检测到TAg5和唾液结合IgE（分别为56％和65％）。从遭受TAg5特异性RNA干扰（RNAi）的果蝇中收获的唾液显示出IgE结合潜力显着降低。TAg5和采采蝇蝇唾液的变应原特性已在经免疫的小鼠中进一步阐明，使用立即的皮肤超敏反应和被动式皮肤过敏试验。总的来说，TAg5被证明是采采蝇蝇唾液过敏原，表明与Antigen5相关的蛋白质不仅在刺痛中而且在食血昆虫中均被表示为功能性过敏原。